A hybrid SAM phospholipid approach to fabricating a ‘free’ supported lipid bilayer

Literature Information

Publication Date 2002-05-01
DOI 10.1039/B200409G
Impact Factor 3.676
Authors

Arwel V. Hughes, Arach Goldar, Michael C. Gerstenberg, Steve J. Roser, Jeremy Bradshaw


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Abstract

Using a combination of self assembly, Langmuir–Blodgett and Langmuir–Schaeffer techniques, we have produced a multilayered film of dimyristoylphosphatidylcholine (DMPC) intended for use as a biomembrane mimic. Neutron reflectivity measurements have revealed that the upper two layers of phospholipid are separated from the silicon substrate and the lower layers by a hydration region approximately 30 Å thick. This layer limits the tethering effect of the substrate such that the phospholipid molecules within the upper bilayer have significant freedom. This freedom is evidenced by the fact that the upper bilayer shows a transition to the Pβ′ ‘ripple’ phase. This phase could not be observed if the phospholipid molecules were significantly constrained by the substrate.

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Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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