3-(3-((2,5-DIOXOPYRROLIDIN-1-YL) OXY)-3-OXOPROPYL)-5,5-DIFLUORO-7,9-DIMETHYL-5H-DIPYRROLO[1,2-C:2',1'-F][1,3,2]DIAZABORININ-4-IUM-5-UIDE(CAS: 146616-66-2)

BODIPY-FL NHS ester (CAS: 146616-66-2) is a water-insoluble, orange-fluorescent compound. It has a molecular weight of approximately 481.4 Da and typically exhibits good reactivity towards primary amines for amide bond formation. It is soluble in organic solvents like DMSO and DMF. The compound tends to form crystalline solids and shows high fluorescence quantum yield, making it useful for labeling biomolecules and imaging applications.

BODIPY-FL NHS ester (CAS: 146616-66-2) is typically synthesized by first protecting the BODIPY core with a hydroxyl group, then converting it to an acetylated derivative. This is followed by esterification with NHS ester to yield the final product. Common solvents include dichloromethane, and the reaction proceeds via a nucleophilic substitution mechanism. Yield is generally around 75-85%, and the reaction is catalyzed by a suitable base or acid depending on the specific step.

BODIPY-FL NHS ester (CAS: 146616-66-2) is widely used in pharmaceutical, biotechnology, and chemical industries. It is particularly valued in the field of fluorescence labeling and imaging, where it is used for conjugating biomolecules, peptides, and proteins. The compound is also employed in the development of fluorescent sensors and as a fluorescent probe in semiconductor and optoelectronic applications due to its excellent photostability and high quantum yield.

When handling BODIPY-FL NHS ester (CAS: 146616-66-2), it is important to wear personal protective equipment (PPE) such as gloves, goggles, and a lab coat. Work should be conducted in a fume hood to minimize exposure to the vapor and aerosols. Spills should be handled with caution using appropriate absorbent materials and followed by thorough cleaning. Always consult the Safety Data Sheet (SDS) before handling the compound for detailed safety information and emergency procedures.