5-Ethynyl-2'-deoxyuridine (EdU)(CAS: 61135-33-9)

5-Ethynyl-2'-deoxyuridine (EdU) is a white or pale yellow crystalline solid with a molecular weight of approximately 240.29 g/mol. It is slightly soluble in water and ethanol. EdU is reactive towards nucleophilic substitution and can form stable complexes with nucleic acids. It is often used in cell labeling and detection due to its ability to incorporate into DNA and RNA.

5-Ethynyl-2'-deoxyuridine (EdU) is typically synthesized via a multi-step process involving the reaction of 2'-deoxyuridine with acetylene and a catalyst such as sodium hydride. The reaction is carried out in a polar solvent like dimethylformamide (DMF) under anhydrous conditions to achieve high yield and selectivity. The process is carefully monitored to ensure optimal conditions for the formation of the ethynyl group.

5-Ethynyl-2'-deoxyuridine (EdU) finds applications primarily in the pharmaceutical and biotechnology industries. It is extensively used in cell biology research for labeling and detecting DNA and RNA synthesis in living cells. Additionally, EdU is used in the formulation of diagnostic kits and in the development of new therapeutic agents.

When handling 5-Ethynyl-2'-deoxyuridine (EdU), it is important to use appropriate personal protective equipment (PPE) such as gloves, goggles, and a lab coat. Work should be conducted in a well-ventilated area or fume hood to minimize inhalation exposure. Spills should be handled according to the safety data sheet (SDS) guidelines, and proper disposal methods should be followed to avoid environmental contamination. Always refer to the SDS for detailed safety information.