Pneumatic nano-sieve for CRISPR-based detection of drug-resistant bacteria

Literature Information

Publication Date 2023-10-18
DOI 10.1039/D3NH00365E
Impact Factor 10.989
Authors

Ruonan Peng, Fengjun Xu, Richard Hailstone, Yujie Men, Ke Du


View Original

Abstract

The increasing prevalence of antibiotic-resistant bacterial infections, particularly methicillin-resistant Staphylococcus aureus (MRSA), presents a significant public health concern. Timely detection of MRSA is crucial to enable prompt medical intervention, limit its spread, and reduce antimicrobial resistance. Here, we introduce a miniaturized nano-sieve device featuring a pneumatically-regulated chamber for highly efficient MRSA purification from human plasma samples. By using packed magnetic beads as a filter and leveraging the deformability of the nano-sieve channel, we achieved an on-chip concentration factor of ∼15-fold for MRSA. We integrated this device with recombinase polymerase amplification (RPA) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas detection system, resulting in an on-chip limit of detection (LOD) of approximately 100 CFU mL−1. This developed approach provides a rapid, precise, and centrifuge-free solution suitable for point-of-care diagnostics, with the potential to significantly improve patient outcomes in resource-limited medical conditions.

Related Literature

Fluorescence spectra of organic dyes in solution: a time dependent multilevel approach

Susanna Monti, Alfonso Pedone, Giacomo Prampolini

2010-12-02 Paper

DOI: 10.1039/C0CP01320J

Si–C-bound alkyl chains on oxide-free Si: towards versatile solution preparation of electronic transport quality monolayers

Avi Lavi, Hagai Cohen, Tatyana Bendikov, Ayelet Vilan, David Cahen

2010-12-03 Communication

DOI: 10.1039/C0CP01445A

Temperature dependence of coarse-grained potentials for liquid hexane

Karim Farah, Aoife Catherine Fogarty, Michael Christian Böhm, Florian Müller-Plathe

2010-12-15 Paper

DOI: 10.1039/C0CP01333A

Demystifying the solvatochromic reversal in Brooker’s merocyanine dye

N. Arul Murugan, Jacob Kongsted, Zilvinas Rinkevicius, Hans Ågren

2010-12-03 Communication

DOI: 10.1039/C0CP01014F

Electronic coherences and vibrational wave-packets in single molecules studied with femtosecond phase-controlled spectroscopy

Richard Hildner, Daan Brinks, Fernando D. Stefani

2011-01-14 Paper

DOI: 10.1039/C0CP02231D

Optical imaging of excited-state tautomerization in single molecules

Anna M. Chizhik, Regina Jäger, Alexey I. Chizhik, Sebastian Bär, Hans-Georg Mack, Marcus Sackrow, Catrinel Stanciu, Alexey Lyubimtsev, Michael Hanack, Alfred J. Meixner

2011-01-14 Perspective

DOI: 10.1039/C0CP02228D

Synthesis and enhanced photocatalytic performance of graphene-Bi2WO6 composite

Erping Gao, Wenzhong Wang, Meng Shang, Jiehui Xu

2010-12-16 Paper

DOI: 10.1039/C0CP01749C

You might also like

Compound Q&A

What precautions should be taken when handling lithium chloride hydrate (1:1:1) (CAS: 16712-20-2)?

When handling lithium chloride hydrate (1:1:1) (CAS: 16712-20-2), it is importan...

16712-20-2Lithium chloride hyd...
Compound Q&A

Is 4-(4H-1,2,4-Triazol-4-yl)piperidine (CAS: 690261-92-8) safe?

4-(4H-1,2,4-Triazol-4-yl)piperidine is generally considered safe for use in phar...

690261-92-84-(4H-1,2,4-Triazol-...
Compound Q&A

How should waste containing 1,3-Thiazole-2-carboxamide (CAS: 16733-85-0) be handled?

Waste containing 1,3-Thiazole-2-carboxamide (CAS: 16733-85-0) should be collecte...

16733-85-01,3-Thiazole-2-carbo...
Compound Q&A

What regulatory guidelines apply to 5-(Difluoromethyl)-2-fluorobenzonitrile (CAS: 934175-58-3)?

5-(Difluoromethyl)-2-fluorobenzonitrile (CAS: 934175-58-3) is subject to regulat...

934175-58-35-(Difluoromethyl)-2...
Compound Q&A

How is Methyl 3-acetamido-2-thiophenecarboxylate (CAS: 22288-79-5) typically synthesized?

Methyl 3-acetamido-2-thiophenecarboxylate can be synthesized by the reaction of ...

22288-79-5Methyl 3-acetamido-2...
Compound Q&A

What is 4-Isoquinolinecarbonitrile (CAS: 34846-65-6)?

4-Isoquinolinecarbonitrile is a chemical compound with the CAS number 34846-65-6...

34846-65-64-Isoquinolinecarbon...
Compound Q&A

How should Methyl 1H-1,2,3-triazole-4-carboxylate (CAS: 877309-59-6) be stored?

Store Methyl 1H-1,2,3-triazole-4-carboxylate (CAS: 877309-59-6) in a cool, dry p...

877309-59-6Methyl 1H-1,2,3-tria...
Compound Q&A

What regulatory guidelines apply to 6-Bromo[1,3]thiazolo[5,4-b]pyridin-2-amine (CAS: 1160791-13-8)?

6-Bromo[1,3]thiazolo[5,4-b]pyridin-2-amine (CAS: 1160791-13-8) is subject to the...

1160791-13-86-Bromo[1,3]thiazolo...
Compound Q&A

Is (2S,3S)-2-Ammonio-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoate (CAS: 23651-95-8) safe?

(2S,3S)-2-Ammonio-3-(3,4-dihydroxyphenyl)-3-hydroxypropanoate (CAS: 23651-95-8) ...

23651-95-8(2S,3S)-2-Ammonio-3-...
Compound Q&A

What are the physical and chemical properties of 7-bromo-3-methyl-3,4-dihydroquinazolin-4-one (CAS: 1293987-84-4)?

7-Bromo-3-methyl-3,4-dihydroquinazolin-4-one is a solid with a crystalline form....

1293987-84-47-bromo-3-methyl-3,4...

Source Journal

Nanoscale Horizons

Nanoscale Horizons
CiteScore: 16.3
Self-citation Rate: 3.4%
Articles per Year: 138

Nanoscale Horizons is a leading journal for the publication of exceptionally high-quality, innovative nanoscience and nanotechnology. The journal places an emphasis on original research that demonstrates a new concept or a new way of thinking (a conceptual advance), rather than primarily reporting technological improvements. However, outstanding articles featuring truly breakthrough developments such as record performance alone may also be published in the journal. For work to be published it must be of significant general interest to our community-spanning readership. Topics covered in the journal include, but are not limited to: Synthesis of nanostructured and nanoscale materials Quantum materials 2D materials Layered materials Layered quantum materials Characterisation of functional nanoscale materials and bio-assemblies Properties of nanoscale materials Self-assembly and molecular organisation Complex hybrid nanostructures Nanocomposites, nanoparticles, nanocrystalline materials, and nanoclusters Nanotubes, molecular nanowires and nanocrystals Molecular nanoscience Nanocatalysis Theoretical modelling Single-molecules Plasmonics Nanoelectronics and molecular electronics Nanophotonics Nanochips, nanosensors, nanofluidics and nanofabrication Carbon-based nanoscale materials and devices Biomimetic materials Nanobiotechnology/bionanomaterials Nanomedicine Regulatory approaches and risk assessment

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.