Kinetics of metal detection by luminescence-based whole-cell biosensors: connecting biosensor response to metal bioavailability, speciation and cell metabolism
Literature Information
Jérôme F. L. Duval, Lorenzo Maffei, Eva Delatour, Marie Zaffino, Christophe Pagnout
Luminescent whole-cell metal biosensors are genetically engineered cells used for the detection of metals in e.g. aqueous solutions. Herein, we detail the quantitative connections between time-response of luminescent bacterial metal sensors and the bioavailability of free and complexed metal species. To that end, we formulate the biophysicochemical dynamics of metal partitioning at a biosensor/solution interface and integrate the required metabolism contribution to cell response. The formalism explains the ways in which cell signal depends on: coupled Eigen kinetics of metal complexation and diffusion of metal species to/from the interface; kinetics of metal excretion, Michaelis–Menten bioaccumulation and ensuing metal depletion from bulk solution; and kinetics of bioluminescence production following intracellular metal sequestration by regulatory metalloproteins. In turn, an expression is derived for the time-dependent cell signal as a function of interrelated (bioavai)lability of metal species and (thermo)dynamic descriptors of extra/intracellular metal complexation. Quantitative criteria are elaborated to identify scenarios where equilibrium modeling of metal speciation is incorrect, bulk metal depletion is operative, metal biouptake kinetics is governed by metal diffusion, or labile metal complexes fully contribute to cell response. Remarkably, in agreement with experiments, the theory predicts time-shifts of bioluminescence peaks with increasing concentration of biosensor and/or metal ligand in solution. We show that these shifts originate from the crosstalk between activation kinetics of cell photoactivity and speciation-dependent kinetics of bulk metal depletion. Overall, the work paves the way for the elaboration of new strategies to exploit the bioluminescence response of metal lux-biosensors at a dynamic level and evaluate metal bioavailability properties in environmental or biological aqueous samples.
Recommended Journals

Main Group Metal Chemistry

Journal of Computer-Aided Molecular Design

Computational Materials Science

Current Medicinal Chemistry

Advanced Materials

Diamond and Related Materials

Construction and Building Materials

Bio-Medical Materials and Engineering

Advanced Powder Technology

Cement and Concrete Composites
Related Literature
Exploring viscosity, polarity and temperature sensitivity of BODIPY-based molecular rotors
Aurimas Vyšniauskas, Ismael López-Duarte, Nicolas Duchemin, Thanh-Truc Vu, Yilei Wu, Ekaterina M. Budynina, Yulia A. Volkova, Eduardo Peña Cabrera, Diana E. Ramírez-Ornelas, Marina K. Kuimova
DOI: 10.1039/C7CP03571C
Acid- and hydrogen-bonding-induced switching between 22-π and 18-π electron conjugations in 2-aminothiazolo[4,5-c]porphycenes
Oriol Planas, Daniel Fernández-Llaneza, Ingrid Nieves, Rubén Ruiz-Gonzalez, Else Lemp, Antonio L. Zanocco, Santi Nonell
DOI: 10.1039/C7CP02938A
New complete assignment of X-ray powder diffraction patterns in graphitic carbon nitride using discrete Fourier transform and direct experimental evidence
Bo-wen Sun, Hong-yu Yu, Yong-jing Yang, Hui-jun Li, Cheng-yu Zhai, Dong-Jin Qian, Meng Chen
DOI: 10.1039/C7CP05242A
A new insight into the thermodynamical criterion for the preparation of semiconductor and metal nanocrystals using a polymerized complexing method
Pingyun Li, Qingqing Wang, Guodong Deng, Xiaode Guo, Wei Jiang, Hongying Liu, Fengsheng Li
DOI: 10.1039/C7CP04097K
Charge transfer induced energy storage in CaZnOS:Mn – insight from experimental and computational spectroscopy
Kurt Lejaeghere
DOI: 10.1039/C7CP00285H
Direct observation of the photoinduced electron transfer processes of bis(4-arylphenylamino benzo)-ortho-carborane using transient absorption spectroscopic measurements
Mi Rang Son, Yang-Jin Cho, So-Yeon Kim, Ho-Jin Son, Sang Ook Kang
DOI: 10.1039/C7CP04505K
Impact of sequential surface-modification of graphene oxide on ice nucleation
Caroline I. Biggs, Christopher Packer, Steven Hindmarsh, Marc Walker, Neil R. Wilson, Jonathan P. Rourke
DOI: 10.1039/C7CP03219F
Theoretical study of the effect of different n-doping elements on band structure and optical gain of GeSn alloys
Wenqi Huang, Hong Yang, Buwen Cheng, Chunlai Xue
DOI: 10.1039/C7CP05840C
You might also like
How should 2-Methylbenzene-1,4-diamine dihydrochloride (CAS: 615-45-2) be stored?
2-Methylbenzene-1,4-diamine dihydrochloride (CAS: 615-45-2) should be stored in ...
Is (1S,4S)-2,5-Diazabicyclo[2.2.1]heptane dihydrobromide (CAS: 132747-20-7) safe?
(1S,4S)-2,5-Diazabicyclo[2.2.1]heptane dihydrobromide is generally considered sa...
What industries use (6-Chloropyridazin-3-YL)methanamine (CAS: 871826-15-2)?
(6-Chloropyridazin-3-YL)methanamine finds applications in the pharmaceutical ind...
What are the main uses of 2-Fluoro-3-methylphenol (CAS: 77772-72-6)?
2-Fluoro-3-methylphenol is primarily used in the synthesis of pharmaceuticals, p...
What precautions should be taken when handling 3-Methoxy-4-nitrobenzonitrile (CAS: 177476-75-4)?
When handling 3-Methoxy-4-nitrobenzonitrile, it is important to wear appropriate...
What precautions should be taken when handling 1,3-Oxazolo[4,5-b]pyridine-2(3H)-thione (CAS: 211949-57-4)?
When handling 1,3-Oxazolo[4,5-b]pyridine-2(3H)-thione (CAS: 211949-57-4), it is ...
What regulatory guidelines apply to 4-Ethynylbenzamide (CAS: 90347-86-7)?
4-Ethynylbenzamide (CAS: 90347-86-7) falls under various regulatory guidelines i...
What are the main uses of 3-(2-Ethylphenyl)-2-thioxo-4-imidazolidinone (CAS: 186822-57-1)?
3-(2-Ethylphenyl)-2-thioxo-4-imidazolidinone is primarily used as an intermediat...
What is (2-Fluoro-6-methoxyphenyl)acetic acid (CAS: 500912-19-6)?
(2-Fluoro-6-methoxyphenyl)acetic acid, also known as 4-fluoro-3-methoxybenzoic a...
What is the market or research trend for 2-[4-(Hydroxymethyl)phenoxy]ethanol (CAS: 102196-18-9)?
Market trends for 2-[4-(Hydroxymethyl)phenoxy]ethanol (CAS: 102196-18-9) indicat...
Source Journal
Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.




![Methyl 2-[5-(3-Phenoxyphenyl)-2H-tetrazol-2-yl]acetate structure Methyl 2-[5-(3-Phenoxyphenyl)-2H-tetrazol-2-yl]acetate structure](https://static.chemtradehub.com/structs/130/1305320-60-8-84b4.webp)