Glucuronan lyases from family PL7 use a Tyr/Tyr syn β-elimination catalytic mechanism for glucuronan breakdown

Literature Information

Publication Date 2023-11-06
DOI 10.1039/D3CC04256A
Impact Factor 6.222
Authors

Marlene Vuillemin, Bo Pilgaard, Emma Kiehn, Folmer Fredslund, Ditte H. Welner, Anne S. Meyer, Finn L. Aachmann, Casper Wilkens


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Abstract

TpPL7A and TpPL7B, members of CAZy family PL7, act as β-glucuronan lyases. TpPL7A diverges by lacking the catalytic histidine, identified as the Brønsted base in PL7 alginate lyases. Our research, including TpPL7A's crystal structure, and mutagenesis studies, reveals a shared syn-β-elimination mechanism with a single tyrosine serving as both base and acid catalyst. This mechanism may extend to subfamily PL7_4 glucuronan lyases.

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