A highly efficient fluorescent probe based on tetrahydroxanthylium–coumarin for the detection of bisulfite in mitochondria
Literature Information
Meng-Xiang Wu, Xue-Rui Wei, Yu-Fang Wei, Ru Sun, Yu-Jie Xu
The addition of bisulfite to an electron-withdrawing double bond has been reported for the design of a bisulfite probe; however, the selectivity of this reaction is usually influenced by an oxidation reaction with hypochlorite. Herein, a probe (probe 1) with tetrahydroxanthylium and coumarin units was designed for the detection of bisulfite. The probe 1 exhibited high selectivity, an obvious colour change from blue to yellow and a large turn-on signal (144-fold enhancement) with green emission towards bisulfite in an acetonitrile-phosphate buffered saline (1 : 9, v/v) solution. In addition, an excellent linear relationship between the fluorescence intensity at 514 nm and bisulfite concentration (0–2 equivalent) was obtained. Moreover, the response time of the probe 1 towards bisulfite was within the order of several seconds, and the detection limit was calculated to be 22.8 nM. The mechanism of the detection reaction was proved by both the 1H NMR analysis and time-dependent density functional theory (TDDFT) calculations. The results of the fluorescence co-localization studies demonstrate that the probe 1 is a mitochondria-targeted fluorescent probe that can be used for the bioimaging of bisulfite in living HeLa cells.
Related Literature
Framework dynamics including computer simulations of the water adsorption isotherm of zeolite Na-MAP
Jörg-Rüdiger Hill, Alan R. Minihan, Erich Wimmer, Chris J. Adams
DOI: 10.1039/B003771K
Intra- and intermolecular interactions in mesogenic cyano compounds
Kayako Hori, Miki Kuribayashi, Mizue Iimuro
DOI: 10.1039/B000567N
Surface processes and electrocatalysis on the Pt(hkl )/Bi-solution interface
Thomas J. Schmidt, Vojislav R. Stamenkovic, Christopher A. Lucas, Nenad M. Markovic, Philip N. Ross Jr.
DOI: 10.1039/B102388H
Structural changes of a Ru(0001) surface under the influence of electrochemical reactions
DOI: 10.1039/B004373G
A computational study of the high voltage LixCoyMn4−yO8 cathode material
J. Spencer Braithwaite, C. Richard A. Catlow, John H. Harding, Julian D. Gale
DOI: 10.1039/B003636F
Electrochemistry of Co–Ru hetero-dinuclear porphyrin complex in a Nafion matrix
Takane Imaoka, Kimihisa Yamamoto
DOI: 10.1039/B105789H
Joint experimental and theoretical study of the poly(styryl sodium) and poly(α-methylstyryl sodium) polymerization/depolymerization
DOI: 10.1039/B105389M
Chiral modified electrodes. Part 2. Marcus behaviour and high enantioselectivity in the photoelectrochemistry at a polymeric [Ru(4-methyl-4′-vinyl-2,2′-bipyridine)3]2+electrode
Christian Felcmann, Gerhard Greiner, Hermann Rau, Michael Wörner
DOI: 10.1039/B002730H
Electronic absorption spectral study of the oxidation of uranium dioxide in chloride melts
Vladimir A. Volkovich, Trevor R. Griffiths, Derek J. Fray, Robert C. Thied
DOI: 10.1039/B004464O
Multicomponent diffusion in systems containing molecules of different size Part 3. Mutual diffusion in the ternary system hexa(ethylene glycol)–di(ethylene glycol)–water‡
Alessandro Vergara, Luigi Paduano, Roberto Sartorio
DOI: 10.1039/B104045F
You might also like
What are the main uses of 1H-Indazole-6-carbonitrile (CAS: 141290-59-7)?
1H-Indazole-6-carbonitrile finds applications in pharmaceuticals, where it serve...
How should waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) be handled?
Waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) should be collecte...
What industries use Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide (CAS: 68291-98-5)?
Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide is primarily used in pharmac...
Are there alternatives to Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxylate (CAS: 741709-66-0) in synthesis?
Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxyla...
How should waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) be handled?
Waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) should be manage...
What is 6-Formyl-2-pyridinecarboxylic acid (CAS: 499214-11-8)?
6-Formyl-2-pyridinecarboxylic acid is an organic compound with the molecular for...
What is the market or research trend for 3-(3,4-dimethoxyphenyl)-2,5-dimethyl-N-(2-morpholin-4-ylethyl)pyrazolo[1,5-a]pyrimidin-7-amine (CAS: 900874-91-1)?
Research trends for this compound indicate a focus on its potential applications...
How is 9H-Tribenzo[b,d,f]azepine (CAS: 29875-73-8) typically synthesized?
9H-Tribenzo[b,d,f]azepine is typically synthesized via a multi-step process invo...
How is 1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxylic acid (CAS: 1797982-51-4) typically synthesized?
1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxyli...
How should waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: 671820-52-3) be handled?
Waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: ...
Source Journal
Analytical Methods

Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development














