A highly efficient fluorescent probe based on tetrahydroxanthylium–coumarin for the detection of bisulfite in mitochondria

Literature Information

Publication Date 2019-07-29
DOI 10.1039/C9AY01355E
Impact Factor 2.896
Authors

Meng-Xiang Wu, Xue-Rui Wei, Yu-Fang Wei, Ru Sun, Yu-Jie Xu


View Original

Abstract

The addition of bisulfite to an electron-withdrawing double bond has been reported for the design of a bisulfite probe; however, the selectivity of this reaction is usually influenced by an oxidation reaction with hypochlorite. Herein, a probe (probe 1) with tetrahydroxanthylium and coumarin units was designed for the detection of bisulfite. The probe 1 exhibited high selectivity, an obvious colour change from blue to yellow and a large turn-on signal (144-fold enhancement) with green emission towards bisulfite in an acetonitrile-phosphate buffered saline (1 : 9, v/v) solution. In addition, an excellent linear relationship between the fluorescence intensity at 514 nm and bisulfite concentration (0–2 equivalent) was obtained. Moreover, the response time of the probe 1 towards bisulfite was within the order of several seconds, and the detection limit was calculated to be 22.8 nM. The mechanism of the detection reaction was proved by both the 1H NMR analysis and time-dependent density functional theory (TDDFT) calculations. The results of the fluorescence co-localization studies demonstrate that the probe 1 is a mitochondria-targeted fluorescent probe that can be used for the bioimaging of bisulfite in living HeLa cells.

Related Literature

Long distance energy transfer in a polymer matrix doped with a perylenedye

Franziska Fennel, Stefan Lochbrunner

2011-01-06 Paper

DOI: 10.1039/C0CP01211D

Fullerene derivative acceptors for high performance polymer solar cells

Youjun He, Yongfang Li

2010-12-22 Perspective

DOI: 10.1039/C0CP01178A

Conversion of methanol over 10-ring zeolites with differing volumes at channel intersections: comparison of TNU-9, IM-5, ZSM-11 and ZSM-5

Francesca Bleken, Wegard Skistad, Katia Barbera, Marina Kustova, Silvia Bordiga, Pablo Beato, Karl Petter Lillerud, Stian Svelle, Unni Olsbye

2010-12-10 Paper

DOI: 10.1039/C0CP01982H

SAXS investigation of a cubic to a sponge (L3) phase transition in self-assembled lipid nanocarriers

Angelina Angelova, Rada Mutafchieva, Sylviane Lesieur, Ulla Vainio, Vasil M. Garamus, Grethe V. Jensen, Jan Skov Pedersen

2010-11-16 Paper

DOI: 10.1039/C0CP01029D

Effect of substituents on redox, spectroscopic and structural properties of conjugated diaryltetrazines—a combined experimental and theoretical study

Ewa Kurach, David Djurado, Jan Rimarčik, Aleksandra Kornet, Marek Wlostowski, Vladimir Lukeš, Jacques Pécaut, Malgorzata Zagorska, Adam Pron

2010-12-09 Paper

DOI: 10.1039/C0CP01553A

Mesoporous carbon capsules as electrode materials in electrochemical double layer capacitors

Shanthi Murali, Daniel R. Dreyer, Patricia Valle-Vigón, Meryl D. Stoller, Yanwu Zhu, Cornelio Morales, Antonio B. Fuertes, Christopher W. Bielawski, Rodney S. Ruoff

2010-12-14 Communication

DOI: 10.1039/C0CP02557G

Mechanistic differences between methanol and dimethyl ethercarbonylation in side pockets and large channels of mordenite

Mercedes Boronat, Cristina Martínez, Avelino Corma

2011-01-19 Paper

DOI: 10.1039/C0CP01996H

Electrochemical control of adsorption dynamics of surface layer proteins on gold

Christian Zafiu, Günter Trettenhahn, Dietmar Pum, Uwe Bernd Sleytr, Wolfgang Kautek

2010-12-22 Paper

DOI: 10.1039/C0CP01099E

You might also like

Compound Q&A

What are the main uses of 1H-Indazole-6-carbonitrile (CAS: 141290-59-7)?

1H-Indazole-6-carbonitrile finds applications in pharmaceuticals, where it serve...

141290-59-71H-Indazole-6-carbon...
Compound Q&A

How should waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) be handled?

Waste containing Dioctyl (2E)-2-butenedioate (CAS: 2997-85-5) should be collecte...

2997-85-5Dioctyl (2E)-2-buten...
Compound Q&A

What industries use Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide (CAS: 68291-98-5)?

Sodium [(1,2-benzoxazol-3-ylmethyl)sulfonyl]azanide is primarily used in pharmac...

68291-98-5Sodium [(1,2-benzoxa...
Compound Q&A

Are there alternatives to Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxylate (CAS: 741709-66-0) in synthesis?

Dimethyl 4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-2,6-pyridinedicarboxyla...

741709-66-0Dimethyl 4-(4,4,5,5-...
Compound Q&A

How should waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) be handled?

Waste containing 2-Fluoro-6-hydrazinopyridine (CAS: 80714-39-2) should be manage...

80714-39-22-Fluoro-6-hydrazino...
Compound Q&A

What is 6-Formyl-2-pyridinecarboxylic acid (CAS: 499214-11-8)?

6-Formyl-2-pyridinecarboxylic acid is an organic compound with the molecular for...

499214-11-86-Formyl-2-pyridinec...
900874-91-13-(3,4-dimethoxyphen...
Compound Q&A

How is 9H-Tribenzo[b,d,f]azepine (CAS: 29875-73-8) typically synthesized?

9H-Tribenzo[b,d,f]azepine is typically synthesized via a multi-step process invo...

29875-73-89H-Tribenzo[b,d,f]az...
Compound Q&A

How is 1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxylic acid (CAS: 1797982-51-4) typically synthesized?

1-Cyclopropyl-7-ethoxy-6-fluoro-8-methoxy-4-oxo-1,4-dihydro-3-quinolinecarboxyli...

1797982-51-41-Cyclopropyl-7-etho...
Compound Q&A

How should waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: 671820-52-3) be handled?

Waste containing Methyl 3-oxo-1,2,3,4-tetrahydro-6-quinoxalinecarboxylate (CAS: ...

671820-52-3Methyl 3-oxo-1,2,3,4...

Source Journal

Analytical Methods

Analytical Methods
CiteScore: 5.1
Self-citation Rate: 3.7%
Articles per Year: 655

Analytical Methods welcomes early applications of new analytical and bioanalytical methods and technology demonstrating the potential for societal impact. We require that methods and technology reported in the journal are sufficiently innovative, robust, accurate, and compared to other available methods for the intended application. Developments with interdisciplinary approaches are particularly welcome. Systems should be proven with suitably complex and analytically challenging samples. We encourage developments within, but not limited to, the following technologies and applications: global health, point-of-care and molecular diagnostics biosensors and bioengineering drug development and pharmaceutical analysis applied microfluidics and nanotechnology omics studies, such as proteomics, metabolomics or glycomics environmental, agricultural and food science neuroscience biochemical and clinical analysis forensic analysis industrial process and method development

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.