Defocused dark-field orientation imaging of single gold microrods on synthetic membranes
Literature Information
Junho Lee, Ji Won Ha
Plasmonic gold microrods (AuMRs) are promising optical probes because of direct observation of their in-plane orientations and motions under an optical microscope. However, our understanding of the optical properties of AuMRs is still very limited to the single particle level, and their out-of-plane orientations cannot be resolved by eye with high accuracy. Herein, we present the scattering properties of 1 μm-long AuMRs under a defocused DF microscope. The characteristic defocused scattering patterns of single AuMRs allowed us to resolve both in-plane and out-of-plane angles of single AuMRs. We further demonstrate the feasibility of using the defocused orientation imaging technique to track rotational motions and out-of-plane angles of single AuMRs on synthetic membranes.
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