Facile fabrication of a 3D electrospun fibrous mat by ice-templating for a tumor spheroid culture

Literature Information

Publication Date 2016-10-14
DOI 10.1039/C6PY01718E
Impact Factor 5.582
Authors

Yanru Li, Lingbo Sun, Hongxia Fu, Xinrui Duan


View Original

Abstract

Electrospinning technology is a simple, convenient, and cost-effective method to fabricate nano/micro fibrous materials. Electrospun fibers are generally collected as a two-dimensional (2D) mat, which lacks a complex and macroscopic three-dimensional (3D) structure. In this work, we fabricated polycaprolactone (PCL) electrospun fibrous materials with small wells (wPCL) by using icy balls as templates. A cooling system was designed and incorporated into the electrospinning setup to achieve our goal. The size and distances between the wells can be easily controlled by a simple water spotting process. When we added cell culture medium droplets into the fabricated wells and placed them upside down, we found that the structure could stabilize the droplets against small mechanical shocks and perturbations, which makes it a good platform for a 3D spheroid culture by the hanging droplet method. Furthermore, the fibrous nature of the electrospun mat ensured the better exchange of O2 and CO2 between the droplet and surrounding environment. We also demonstrated that the cubic and triangle shaped 3D electrospun fibrous mat can be fabricated using a similar methodology. Our results indicate that electrospinning technology involving ice-templated collection is a mild and straightforward way to fabricate 3D electrospun materials and can be readily used for a 3D cell culture.

Related Literature

When amine-based conducting polymers meet Au nanoparticles: suppressing H2 evolution and promoting the selective electroreduction of CO2 to CO at low overpotentials

Jialei Yu, Han Zhu, Jiace Hao, Huilin Li, Huining Li, Shuanglong Lu, Mingliang Du

2020-12-23 Paper

DOI: 10.1039/D0SE01848A

A facile preparation of PEO–LiClO4–fumed SiO2 composite solid-state electrolyte with improved electrochemical performance for lithium-metal batteries

Ruyan Lei, Yanping Yang, Chenjuan Yu, Yinsi Xu, Yuanzhuo Li, Jun Li

2021-01-28 Paper

DOI: 10.1039/D1SE00038A

Pt atoms on doped carbon nanosheets with ultrahigh N content as a superior bifunctional catalyst for hydrogen evolution/oxidation

Cheng Jiang, Ping Li, Qi Feng, Zhi liang Zhao, Keguang Yao, Jiantao Fan, Hui Li, Haijiang Wang

2020-11-06 Paper

DOI: 10.1039/D0SE01516D

Improving the electrocatalytic performance of sustainable Co/carbon materials for the oxygen evolution reaction by ultrasound and microwave assisted synthesis

Alessio Zuliani, Manuel Cano, Federica Calsolaro, Alain R. Puente Santiago, Juan J. Giner-Casares, Enrique Rodríguez-Castellón, Gloria Berlier, Giancarlo Cravotto, Katia Martina

2020-12-11 Paper

DOI: 10.1039/D0SE01505A

Crystal size-controlled growth of bismuth vanadate for highly efficient solar water oxidation

Qi Qin, Qian Cai, Wei Liu

2021-01-08 Paper

DOI: 10.1039/D0SE01642J

You might also like

Compound Q&A

What precautions should be taken when handling 2-Methyl-2-propanyl 5-amino-2-thiophenecarboxylate (CAS: 1498311-57-1)?

When handling 2-Methyl-2-propanyl 5-amino-2-thiophenecarboxylate (CAS: 1498311-5...

1498311-57-12-Methyl-2-propanyl ...
Compound Q&A

What are the physical and chemical properties of 5-Bromo-1,2-dichloro-3-fluorobenzene (CAS: 1000572-93-9)?

5-Bromo-1,2-dichloro-3-fluorobenzene (CAS: 1000572-93-9) is a crystalline solid ...

1000572-93-95-Bromo-1,2-dichloro...
Compound Q&A

How should (2R)-2-Amino-2-(4-bromophenyl)ethanol (CAS: 354153-64-3) be stored?

(2R)-2-Amino-2-(4-bromophenyl)ethanol (CAS: 354153-64-3) should be stored in a c...

354153-64-3(2R)-2-Amino-2-(4-br...
Compound Q&A

What regulatory guidelines apply to Methyl 4-(aminomethyl)tetrahydro-2H-pyran-4-carboxylate hydrochloride (CAS: 362707-24-2)?

Methyl 4-(aminomethyl)tetrahydro-2H-pyran-4-carboxylate hydrochloride (CAS: 3627...

362707-24-2Methyl 4-(aminomethy...
Compound Q&A

What are the main uses of 1,4-dimethyl-1H-pyrazole-5-sulfonyl chloride (CAS: 1174834-52-6)?

1,4-Dimethyl-1H-pyrazole-5-sulfonyl chloride is primarily used as an intermediat...

1174834-52-61,4-dimethyl-1H-pyra...
Compound Q&A

Is Dinaphtho[1,2-b:2',1'-d]furan (CAS: 239-69-0) safe?

Dinaphtho[1,2-b:2',1'-d]furan is generally safe when handled with appropriate pe...

239-69-0Dinaphtho[1,2-b:2',1...
Compound Q&A

What is the market or research trend for 7-Methyl-7,9-dihydro-1H-purine-2,6,8(3H)-trione (CAS: 612-37-3)?

The market for 7-Methyl-7,9-dihydro-1H-purine-2,6,8(3H)-trione (CAS: 612-37-3) i...

612-37-37-Methyl-7,9-dihydro...
Compound Q&A

What are the physical and chemical properties of 2-(4-Chlorophenyl)malonaldehyde (CAS: 205676-17-1)?

2-(4-Chlorophenyl)malonaldehyde (CAS: 205676-17-1) is a colorless or light yello...

205676-17-12-(4-Chlorophenyl)ma...
Compound Q&A

How is 2-Methylchrysene (CAS: 3351-32-4) typically synthesized?

2-Methylchrysene (CAS: 3351-32-4) is typically synthesized via the reaction of c...

3351-32-42-Methylchrysene
Compound Q&A

Is N-(6-aminopyrimidin-4-yl)acetamide (CAS: 89533-23-3) safe?

N-(6-aminopyrimidin-4-yl)acetamide (CAS: 89533-23-3) is generally considered saf...

89533-23-3N-(6-aminopyrimidin-...

Source Journal

Polymer Chemistry

Polymer Chemistry
CiteScore: 8.6
Self-citation Rate: 7.3%
Articles per Year: 457

Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.