Predicting efficacies of anticancer drugs using single cell HaloChip assay
Literature Information
Publication Date
2016-02-29
DOI
10.1039/C5AN02564H
Impact Factor
4.616
Authors
Yong Qiao, Jincui An
View Original
Abstract
Single cell halo assay (HaloChip) is used to quantify DNA repair ability and predict the efficacy of anticancer drugs. After exposure to drugs, cells are patterned onto a substrate to form an ordered single cell array, then embedded inside an agarose gel, and fluorescently stained to generate a characteristic halo surrounding each cell. The extent of DNA repair is quantified by using a relative nuclear diffusion factor (rNDF) derived from the surface areas of nuclei and halos. Several repair-competent and repair-deficient cell lines have been used to validate this method. Drug-inhibitor combinations are also tested in the context of synthetic lethality of chemotherapy, where the use of a repair inhibitor potentiates the effects of DNA damaging agents. This paper highlights the important role of HaloChip in quantifying DNA repair ability, which provides the diagnostic utility to enhance the efficacies of anticancer drugs.
Recommended Journals
European Journal of Organic Chemistry
Journal of Enzyme inhibition and Medicinal Chemistry
Coloration Technology
Physical Chemistry Chemical Physics
CrystEngComm
Journal of Medical Biochemistry
Angewandte Chemie International Edition
Environmental Toxicology and Pharmacology
Contact Lens & Anterior Eye
Faraday Discussions
Related Literature
An orbitally adapted push–pull template for N2 activation and reduction to diazene-diide
David Specklin, Marie-Christine Boegli, Anaïs Coffinet, Léon Escomel, Laure Vendier, Mary Grellier, Antoine Simonneau
2023-11-21
Edge Article
DOI: 10.1039/D3SC04390H
Oxidative cleavage of ketoximes to ketones using photoexcited nitroarenes
Lucas T. Göttemann, Stefan Wiesler, Richmond Sarpong
2023-11-24
Edge Article
DOI: 10.1039/D3SC05414D
The phenomenon of “dead” metal in heterogeneous catalysis: opportunities for increasing the efficiency of carbon-supported metal catalysts
Roman M. Mironenko
2023-11-20
Review Article
DOI: 10.1039/D3SC04691E
An expeditive and green chemo-enzymatic route to diester sinapoyl-l-malate analogues: sustainable bioinspired and biosourced UV filters and molecular heaters
Benjamin Rioux, Louis M. M. Mouterde, Jimmy Alarcan, Matthias J. A. Vink, Jack M. Woolley, Aurélien A. M. Peru, Matthieu M. Mention, Fanny Brunissen, Giel Berden, Jos Oomens, Albert Braeuning, Florent Allais
2023-11-21
Edge Article
DOI: 10.1039/D3SC04836E
Photocatalytic (3 + 2) dipolar cycloadditions of aziridines driven by visible-light
Daniele Mazzarella, Tommaso Bortolato, Giorgio Pelosi, Luca Dell'Amico
2023-11-29
Edge Article
DOI: 10.1039/D3SC05997A
Computationally guided bioengineering of the active site, substrate access pathway, and water channels of thermostable cytochrome P450, CYP175A1, for catalyzing the alkane hydroxylation reaction
Mohd Taher, Kshatresh Dutta Dubey, Shyamalava Mazumdar
2023-12-04
Edge Article
DOI: 10.1039/D3SC02857G
Manipulating the crystal plane angle within the primary particle arrangement for the radial ordered structure in a Ni-rich cathode
Ting Chen, Chuyao Wen, Chen Wu, Lang Qiu, Zhenguo Wu, Jiayang Li, Yanfang Zhu, Haoyu Li, Qingquan Kong, Yang Song, Fang Wan, Mingzhe Chen, Ismael Saadoune, Benhe Zhong, Shixue Dou, Yao Xiao
2023-11-27
Edge Article
DOI: 10.1039/D3SC05461F
Dynamic sampling of liquid metal structures for theoretical studies on catalysis
Charlie Ruffman, Krista G. Steenbergen, Anna L. Garden, Nicola Gaston
2023-11-29
Edge Article
DOI: 10.1039/D3SC04416E
A salt-concentrated electrolyte for aqueous ammonium-ion hybrid batteries
Jianming Meng, Jing Wang, Peng Hei, Chang Liu, Mengxue Li, Yulai Lin
2023-11-29
Edge Article
DOI: 10.1039/D3SC05318K
(C5H6.16N2Cl0.84)(IO2Cl2): a birefringent crystal featuring unprecedented (IO2Cl2)− anions and π-conjugated organic cations
Chun-Li Hu
2023-11-27
Edge Article
DOI: 10.1039/D3SC05770D
You might also like
Compound Q&A
What precautions should be taken when handling 4-(2-Furylmethyl)thiomorpholine 1,1-dioxide (CAS: 79206-94-3)?
When handling 4-(2-Furylmethyl)thiomorpholine 1,1-dioxide (CAS: 79206-94-3), it ...
79206-94-34-(2-Furylmethyl)thi...
Compound Q&A
What precautions should be taken when handling 4-Chloro-N-[2-(4-morpholinyl)ethyl]benzamide (CAS: 71320-77-9)?
When handling 4-Chloro-N-[2-(4-morpholinyl)ethyl]benzamide (CAS: 71320-77-9), it...
71320-77-94-Chloro-N-[2-(4-mor...
Compound Q&A
How should waste containing 2-[2-(2-Methoxyethoxy)ethoxy]ethyl 4-methylbenzenesulfonate (CAS: 62921-74-8) be handled?
Waste containing this compound (CAS: 62921-74-8) should be handled according to ...
62921-74-82-[2-(2-Methoxyethox...
Compound Q&A
How should waste containing (S)-Methyl 2-amino-3-cyclohexylpropanoate be handled?
Waste containing (S)-Methyl 2-amino-3-cyclohexylpropanoate should be collected i...
40056-18-6(S)-Methyl 2-amino-3...
Compound Q&A
How is 5-({4-[(2S,4R)-4-Hydroxy-2-methyltetrahydro-2H-pyran-4-yl]-2-thienyl}sulfanyl)-1-methyl-1,3-dihydro-2H-indol-2-one (CAS: 166882-70-8) typically synthesized?
This compound can be synthesized using a multi-step process involving the conjug...
166882-70-85-({4-[(2S,4R)-4-Hyd...
Compound Q&A
Are there alternatives to (2E)-3-(3,4-Dichlorophenyl)acrylic acid (CAS: 7312-27-8) in synthesis?
There are several alternatives to (2E)-3-(3,4-Dichlorophenyl)acrylic acid in syn...
7312-27-8(2E)-3-(3,4-Dichloro...
Compound Q&A
How should Ethyl 6-(2-nitrophenyl)imidazo[2,1-b][1,3]thiazole-3-carboxylate (CAS: 925437-84-9) be stored?
Ethyl 6-(2-nitrophenyl)imidazo[2,1-b][1,3]thiazole-3-carboxylate (CAS: 925437-84...
925437-84-9Ethyl 6-(2-nitrophen...
Compound Q&A
How should waste containing 2-(1,3-Thiazol-2-yl)ethanamine (CAS: 18453-07-1) be handled?
Waste containing 2-(1,3-Thiazol-2-yl)ethanamine (CAS: 18453-07-1) should be coll...
18453-07-12-(1,3-Thiazol-2-yl)...
Compound Q&A
How is Methyl 5-iodo-2-methylbenzoate (CAS: 103440-54-6) typically synthesized?
Methyl 5-iodo-2-methylbenzoate can be synthesized through the iodination of meth...
103440-54-6Methyl 5-iodo-2-meth...
Compound Q&A
How is 5-Chloro[1,2,4]triazolo[1,5-a]pyridine (CAS: 1427399-34-5) typically synthesized?
5-Chloro[1,2,4]triazolo[1,5-a]pyridine is commonly synthesized via the condensat...
1427399-34-55-Chloro[1,2,4]triaz...
Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
![1-[3-(4-Morpholinylsulfonyl)phenyl]methanamine structure](https://static.chemtradehub.com/structs/933/933989-32-3-51af.webp "1-[3-(4-Morpholinylsulfonyl)phenyl]methanamine structure")
Recommended Suppliers
Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.