1-(3-Fluorophenyl)-1H-1,2,4-triazole-3-carboxylic acid
CAS Number:
1292369-51-7
Molecular Formula:
C9H6FN3O2
Pre-equilibration kinetic size-exclusion chromatography with mass spectrometry detection (peKSEC-MS) for label-free solution-based kinetic analysis of protein–small molecule interactions
Literature Information
Publication Date
2015-01-07
DOI
10.1039/C4AN02232G
Impact Factor
4.616
Authors
Jiayin Bao, Svetlana M. Krylova, Leonid T. Cherney, J. C. Yves Le Blanc, Patrick Pribil, Philip E. Johnson, Derek J. Wilson, Sergey N. Krylov
View Original
Abstract
Here we introduce pre-equilibration kinetic size-exclusion chromatography with mass-spectrometry detection (peKSEC-MS), which is a label-free solution-based kinetic approach for characterizing non-covalent protein–small molecule interactions. In this method, a protein and a small molecule are mixed outside the column and incubated to approach equilibrium. The equilibrium mixture is then introduced into the SEC column to initiate the dissociation process by separating small molecules from the complex inside the column. A numerical model of a 1-dimensional separation was constructed to simulate mass chromatograms of the small molecule for varying rate constants of binding.
Related Literature
A Prussian blue analog-based copper–aluminum layered double hydroxide for cesium removal from water: fabrication, density functional theory-based molecular modeling, and the adsorption mechanism
Meng Xia, Xinyao Liu, Zhaorong Shang, Fuqiang Fan
2023-12-15
Paper
DOI: 10.1039/D3CP03879C
Reinforcement learning-based control for the thermal management of the battery and occupant compartments of electric vehicles
Jianglu Huang, Donggang Zhao, Yu Zhao
2024-01-03
Paper
DOI: 10.1039/D3SE01403G
Design of J-aggregates-like oligomers built from squaraine dyes exhibiting transparency in the visible regime and high fluorescence quantum yield in the NIR region
Margarita Bužančić Milosavljević
2023-12-05
Paper
DOI: 10.1039/D3CP05291E
Unravelling the band splitting origin in bulk and 2D distorted α-CsPbI3 perovskite
Safieh Nazari, Fatemeh Mohammad Dezashibi, Nadia Babaei Bidmeshki
2023-12-05
Paper
DOI: 10.1039/D3CP04558G
Delayed photodissociation of the tin cluster Sn22−
Alexander Jankowski, Paul Fischer, Klavs Hansen, Lutz Schweikhard
2023-12-15
Paper
DOI: 10.1039/D3CP04476A
Predicting the pair correlation functions of silicate and borosilicate glasses using machine learning
Kumar Ayush, Pooja Sahu, Sk. Musharaf Ali, Tarak K. Patra
2023-12-04
Paper
DOI: 10.1039/D3CP05136F
Reaction mechanism of the ethynylation of formaldehyde on copper terminated Cu2O(100) surfaces: a DFT study
2023-11-29
Paper
DOI: 10.1039/D3CP03903J
Integrative electrochemical and biological catalysis for the mild and efficient utilization of renewable electricity and carbon resources
Licheng Liu, Deepak Pant
2023-12-06
Review Article
DOI: 10.1039/D3SE00876B
Theoretical investigation on the ligands constructed from phenanthroline and five-membered N-heterocyclic rings for bonding and separation properties of Am(iii) and Eu(iii)
Shouqiang Wu, An Yong Li
2023-12-12
Paper
DOI: 10.1039/D3CP05101C
Energy transfer from two luteins to chlorophylls in light-harvesting complex II study by using exciton models with phase correction
Jiarui Li, Tao Zeng, Zexing Qu, Yu Zhai, Hui Li
2023-12-04
Paper
DOI: 10.1039/D3CP05278H
You might also like
Compound Q&A
How should waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3) be handled?
Waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3...
898825-89-3N-Methoxy-N-methyl-1...
Compound Q&A
How should N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine (CAS: 1318338-47-4) be stored?
N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine should be stored in a tightly sealed c...
1318338-47-4N-(4-Biphenylyl)dibe...
Compound Q&A
What is the market or research trend for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1)?
The market for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1) is...
1713-07-13-Acetamido-5-amino-...
Compound Q&A
How should Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) be stored?
Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) ...
61820-03-9Benzyl 2-O-acetyl-3,...
Compound Q&A
What regulatory guidelines apply to 2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3)?
2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3) is regulated under the Glob...
438050-52-32-Ethylpiperazine di...
Compound Q&A
What regulatory guidelines apply to 1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 119462-56-5)?
1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 11946...
119462-56-51,1'-[1,3-Phenyleneb...
Compound Q&A
Are there alternatives to 5-Fluoro-2-(1-pyrrolidinyl)pyridine (CAS: 1287217-79-1) in synthesis?
Several alternatives can be used in the synthesis of 5-Fluoro-2-(1-pyrrolidinyl)...
1287217-79-15-Fluoro-2-(1-pyrrol...
Compound Q&A
What precautions should be taken when handling 1-((2R,3R,4R,5R)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-hydroxy-3-methoxytetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (CAS: 153631-19-7)?
Proper personal protective equipment (PPE) must be worn when handling this compo...
153631-19-71-((2R,3R,4R,5R)-5-(...
Compound Q&A
What precautions should be taken when handling 6-Bromoimidazo[1,2-a]pyridin-8-amine (CAS: 676371-00-9)?
When handling 6-Bromoimidazo[1,2-a]pyridin-8-amine, it is important to wear appr...
676371-00-96-Bromoimidazo[1,2-a...
Compound Q&A
Are there alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochloride (CAS: 1049740-22-8) in synthesis?
Alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochlo...
1049740-22-8(2S,4R)-4-(4-Nitrobe...
Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
N-(5-Fluoro-2-oxo-2,3-dihydro-4-pyrimidinyl)benzamide
CAS Number:
10357-07-0
Molecular Formula:
C11H8FN3O2
1-(3,4-Dichlorophenyl)-1H-pyrazole-3-carboxylic acid
CAS Number:
1152949-12-6
Molecular Formula:
C10H6Cl2N2O2
Recommended Suppliers
Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.