Protic equilibria as the key factor of quercetin emission in solution. Relevance to biochemical and analytical studies‡

Literature Information

Publication Date 2011-03-09
DOI 10.1039/C0CP00714E
Impact Factor 3.676
Authors

Christine Lapouge, Jean-Paul Cornard


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Abstract

A detailed spectrofluorimetric study on quercetin in aqueous solution proves that its anionic forms are responsible for a strong fluorescence enhancement observed at pH > 6. Anion fluorescence is also observed in organic solvents with strong hydrogen bond acceptor properties. The results provide a new interpretation of biophysical and analytical literature data where the fluorescence of the anionic forms of quercetin has never been explicitly taken into account. In particular, comparison with published binding studies strongly suggests that quercetin deprotonation and consequent fluorescence enhancement takes place when the flavonoid binds to several biological macromolecules. This observation can be very helpful in the understanding at a molecular level of the interaction between quercetin and the biomolecule.

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Contents list

Front/Back Matter

DOI: 10.1039/C5CP90095F

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Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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