Redox properties and catalytic activity of surface-bound human sulfite oxidase studied by a combined surface enhanced resonance Raman spectroscopic and electrochemical approach

Literature Information

Publication Date 2010-05-26
DOI 10.1039/B927226G
Impact Factor 3.676
Authors

Murat Sezer, Roberto Spricigo, Tillmann Utesch, Diego Millo, Silke Leimkuehler, Maria A. Mroginski, Ulla Wollenberger, Peter Hildebrandt, Inez M. Weidinger


View Original

Abstract

Human sulfite oxidase (hSO) was immobilised on SAM-coated silver electrodes under preservation of the native heme pocket structure of the cytochrome b5 (Cyt b5) domain and the functionality of the enzyme. The redox properties and catalytic activity of the entire enzyme were studied by surface enhanced resonance Raman (SERR) spectroscopy and cyclic voltammetry (CV) and compared to the isolated heme domain when possible. It is shown that heterogeneous electron transfer and catalytic activity of hSO sensitively depend on the local environment of the enzyme. Increasing the ionic strength of the buffer solution leads to an increase of the heterogeneous electron transfer rate from 17 s−1 to 440 s−1 for hSO as determined by SERR spectroscopy. CV measurements demonstrate an increase of the apparent turnover rate for the immobilised hSO from 0.85 s−1 in 100 mM buffer to 5.26 s−1 in 750 mM buffer. We suggest that both effects originate from the increased mobility of the surface-bound enzyme with increasing ionic strength. In agreement with surface potential calculations we propose that at high ionic strength the enzyme is immobilised via the dimerisation domain to the SAM surface. The flexible loop region connecting the Moco and the Cyt b5 domain allows alternating contact with the Moco interaction site and the SAM surface, thereby promoting the sequential intramolecular and heterogeneous electron transfer from Moco via Cyt b5 to the electrode. At lower ionic strength, the contact time of the Cyt b5 domain with the SAM surface is longer, corresponding to a slower overall electron transfer process.

Related Literature

Defect engineering: the role of cationic vacancies in photocatalysis and electrocatalysis

Wenming Ding, Shengbo Yuan, Yang Yang, Xiaoman Li, Min Luo

2023-10-17 Review Article

DOI: 10.1039/D3TA04947G

Topological insulator bismuth selenide with a unique cloud-like hollow structure as a bidirectional electrocatalyst for robust lithium–sulfur batteries

Mincai Zhao, Junjie Fu, Daoping Cai, Chaoqi Zhang, Yinggan Zhang, Baisheng Sa, Qidi Chen, Hongbing Zhan

2023-10-20 Paper

DOI: 10.1039/D3TA04930B

Benzimidazole-modified organosilane functionalized silica nanoparticles as a ‘turn-off’ fluorescent probe for highly selective Cu2+ ion detection: unravelling logic gate behaviour and molecular docking studies

Gurjaspreet Singh, Mohit, Akshpreet Singh, Priyanka, Sumesh Khurana, Mithun, K. N. Singh, Jasamrit Nayyar, Brij Mohan

2023-12-22 Paper

DOI: 10.1039/D3NJ05199D

Iodine-loaded ZIF-7-coated cotton substrates show sustained iodine release as effective antibacterial textiles

Donya Mohammadi Amidi, Kamran Akhbari

2023-12-13 Paper

DOI: 10.1039/D3NJ05198F

Cu2ZnSnS4 monograin layer solar cells for flexible photovoltaic applications

Marit Kauk-Kuusik, Kristi Timmo, Maris Pilvet, Katri Muska, Mati Danilson, Jüri Krustok, Raavo Josepson, Valdek Mikli, Maarja Grossberg-Kuusk

2023-10-23 Review Article

DOI: 10.1039/D3TA04541B

Selective production of γ-valerolactone from biomass-derived levulinic acid over a Ni/CMK-3 catalyst

Rui Zhang, Xishang Song, Han Wu, Yunqi Zhai, Yina Qiao, Zhihao Yu, Jian Xiong, Xuebin Lu

2023-12-23 Paper

DOI: 10.1039/D3NJ04771G

Front cover

2024-01-29 Cover

DOI: 10.1039/D4NJ90014F

Removal of total chromium in wastewater via simultaneous photocatalysis and adsorption using calcium silicate hydrate-based composites

Min Liu, Qi Liu, Xue-Ting Jin, Ya-Chen Zou, Di-Ning Li, Pan Feng, Yang-Hui Luo

2023-10-19 Paper

DOI: 10.1039/D3TA05384A

You might also like

Compound Q&A

How should waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3) be handled?

Waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3...

898825-89-3N-Methoxy-N-methyl-1...
Compound Q&A

How should N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine (CAS: 1318338-47-4) be stored?

N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine should be stored in a tightly sealed c...

1318338-47-4N-(4-Biphenylyl)dibe...
Compound Q&A

What is the market or research trend for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1)?

The market for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1) is...

1713-07-13-Acetamido-5-amino-...
Compound Q&A

How should Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) be stored?

Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) ...

61820-03-9Benzyl 2-O-acetyl-3,...
Compound Q&A

What regulatory guidelines apply to 2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3)?

2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3) is regulated under the Glob...

438050-52-32-Ethylpiperazine di...
Compound Q&A

What regulatory guidelines apply to 1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 119462-56-5)?

1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 11946...

119462-56-51,1'-[1,3-Phenyleneb...
Compound Q&A

Are there alternatives to 5-Fluoro-2-(1-pyrrolidinyl)pyridine (CAS: 1287217-79-1) in synthesis?

Several alternatives can be used in the synthesis of 5-Fluoro-2-(1-pyrrolidinyl)...

1287217-79-15-Fluoro-2-(1-pyrrol...
Compound Q&A

What precautions should be taken when handling 6-Bromoimidazo[1,2-a]pyridin-8-amine (CAS: 676371-00-9)?

When handling 6-Bromoimidazo[1,2-a]pyridin-8-amine, it is important to wear appr...

676371-00-96-Bromoimidazo[1,2-a...
Compound Q&A

Are there alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochloride (CAS: 1049740-22-8) in synthesis?

Alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochlo...

1049740-22-8(2S,4R)-4-(4-Nitrobe...

Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.