In vivo recognition of Bacillus subtilis by desorption electrospray ionizationmass spectrometry (DESI-MS)
Literature Information
Publication Date
2009-03-16
DOI
10.1039/B900069K
Impact Factor
4.616
Authors
Yishu Song, Nari Talaty, Kirill Datsenko, Barry L. Wanner, R. Graham Cooks
View Original
Abstract
Desorption electrospray ionization mass spectrometry (DESI-MS) of culture of the bacterium Bacillus subtilis as a biofilm growing on agar nutrient gives simple, high quality mass spectra dominated in both the positive and negative ion modes by signals due to the cyclic lipopeptide, Surfactin(C15). This in vivo experiment, performed by direct analysis of untreated microorganism samples under ambient conditions, allows rapid identification of this microorganism and the antibiotics that it produces. The result is suggestive of the capabilities of DESI-MS for in vivo microorganism characterization in general and for monitoring fermentation processes for the production of antibiotics and other biochemicals.
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1-(Hexopyranosyloxy)-4a,5-dihydroxy-7-methyl-1,4a,5,6,7,7a-hexahydrocyclopenta[c]pyran-7-yl 3-phenylacrylate
CAS Number:
19210-12-9
Molecular Formula:
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