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Electrophoretic field gradient focusing with on-column detection by fluorescence quenching
Literature Information
Publication Date
2008-12-24
DOI
10.1039/B815446E
Impact Factor
4.616
Authors
Richard J. Ansell, Yating Wang, Cornelius F. Ivory, Jeffrey N. Keen, John B. C. Findlay
View Original
Abstract
Native, uncoloured, proteins can be focused in a column containing a fluorescent packing material, using hydrodynamic flow and a counteracting non-linear electric field, and imaged along the length of the channel by fluorescence quenching.
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