Electrophoretic field gradient focusing with on-column detection by fluorescence quenching

Literature Information

Publication Date 2008-12-24
DOI 10.1039/B815446E
Impact Factor 4.616
Authors

Richard J. Ansell, Yating Wang, Cornelius F. Ivory, Jeffrey N. Keen, John B. C. Findlay


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Abstract

Native, uncoloured, proteins can be focused in a column containing a fluorescent packing material, using hydrodynamic flow and a counteracting non-linear electric field, and imaged along the length of the channel by fluorescence quenching.

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