Following a protein kinase activity using a field-effect transistor device

Literature Information

Publication Date 2007-07-30
DOI 10.1039/B707677K
Impact Factor 6.222
Authors

Ronit Freeman, Ron Gill, Itamar Willner


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Abstract

The specific phosphorylation of a peptide-functionalized ion-sensitive field-effect transistor device by casein kinase II in the presence of ATP enables the electronic readout of the protein kinase activity; treatment of the phosphorylated surface with alkaline phosphatase results in the regeneration of the active sensing surface.

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Chemical Communications

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