Biocatalytic deuterium- and hydrogen-transfer using over-expressed ADH-‘A’: enhanced stereoselectivity and 2H-labeled chiral alcohols

Literature Information

Publication Date 2006-04-11
DOI 10.1039/B602487D
Impact Factor 6.222
Authors

Christian C. Gruber, Tina M. Poessl, Frank Niehaus, Juergen Eck, Reinhold Oehrlein, Andreas Hafner


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Abstract

Employing the over-expressed highly organic solvent tolerant alcohol dehydrogenase ADH-‘A’ from Rhodococcus ruber DSM 44541, versatile building blocks, which were not accessible by the wild type catalyst, were obtained in > 99% e.e.; furthermore, employing d8-2-propanol as deuterium source, stereoselective biocatalytic deuterium transfer was made feasible to furnish enantiopure deuterium labeled sec-alcohols on a preparative scale employing a single enzyme.

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