An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity
Literature Information
Publication Date
2016-08-08
DOI
10.1039/C6AN01073C
Impact Factor
4.616
Authors
Sara M. Elkashef, Mark Sutherland, Laurence H. Patterson, Paul M. Loadman, Robert A. Falconer
View Original
Abstract
The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer. Having improved the chemical synthesis and purification of the fluorescently-labelled DMB–DP3 acceptor, we report optimisation and validation of a highly sensitive cell-free high-throughput HPLC-based assay for assessment of human polysialyltransferase activity.
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