![6-Nitro-2-(4-nitrophenyl)imidazo[1,2-a]pyridine structure](https://static.chemtradehub.com/structs/118/118000-57-0-cb82.webp "6-Nitro-2-(4-nitrophenyl)imidazo[1,2-a]pyridine structure")
A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish
Literature Information
Publication Date
2016-06-15
DOI
10.1039/C6AN00905K
Impact Factor
4.616
Authors
Wei Hu, Lingyu Zeng, Yanying Wang, Zhihong Liu, Xiaoxue Ye, Chunya Li
View Original
Abstract
Using 6-hydroxyl-quinoline-2-benzothiazole (HQB) as a two-photon fluorophore and tert-butyldiphenylsilyl as a recognition domain for F−, a ratiometric two-photon fluorescent fluoride probe, QF, was synthesized and fully characterized. QF displays both one- and two-photon ratiometric responses towards fluoride ions in aqueous solution. QF was enabled to detect exogenous fluoride ions in living cells by a ratiometric method. Two-photon microscopic imaging of fluoride ions in living HeLa cells and zebrafish has also been achieved. QF has been demonstrated to be an excellent fluorescent probe with high selectivity, low cytotoxicity and good photostability.
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