Design and application of an open tubular capillary reactor for solid-phase permethylation of glycans in glycoprotein

Literature Information

Publication Date 2014-12-24
DOI 10.1039/C4AN01045K
Impact Factor 4.616
Authors

Xiaodi Gao, Lingyi Zhang, Weibing Zhang, Liang Zhao


View Original

Abstract

The permethylation derivatization method for structural analysis of glycans is important for characterizing glycoproteins in the study of glycomics. An open tubular capillary reactor coated with NaOH was designed and constructed for solid-phase permethylation of glycans in glycoproteins. The flow rate, capillary length, and inner diameter of the reactor were optimized. The permethylation rate of the model sample β-cyclodextrin reached 81% with a flow rate of 1 μL min−1 in a 32 cm long capillary reactor (i.d. 500 μm). A trace amount of mucin O-glycans was permethylated by the open tubular reactor under low pressure without interference from the freezing of DMSO. Analysis indicates that using this open tubular reactor is a fast, convenient, and efficient method for the permethylation of protein O-glycan.

Related Literature

Contents list

2023-11-14 Front/Back Matter

DOI: 10.1039/D3TA90246C

Green synthesis of fluorescent carbon quantum dots from bagasse: inhibition of calcium sulphate scales

Fangming Yang, Duanzhi Li, Zhihao Chen, Wenzhong Yang

2023-12-22 Paper

DOI: 10.1039/D3NJ05155B

Inside back cover

2023-11-14 Cover

DOI: 10.1039/D3TA90247A

Chemical capacitance measurements reveal the impact of oxygen vacancies on the charge curve of LiNi0.5Mn1.5O4−δ thin films

Andreas E. Bumberger, Sergej Ražnjević, Zaoli Zhang, Gernot Friedbacher, Juergen Fleig

2023-10-16 Paper

DOI: 10.1039/D3TA05086F

Benzimidazole-modified organosilane functionalized silica nanoparticles as a ‘turn-off’ fluorescent probe for highly selective Cu2+ ion detection: unravelling logic gate behaviour and molecular docking studies

Gurjaspreet Singh, Mohit, Akshpreet Singh, Priyanka, Sumesh Khurana, Mithun, K. N. Singh, Jasamrit Nayyar, Brij Mohan

2023-12-22 Paper

DOI: 10.1039/D3NJ05199D

Engineering yeast for the production of plant terpenoids using synthetic biology approaches

Jean-Alexandre Bureau, Magdalena Escobar Oliva, Yueming Dong, Codruta Ignea

2023-07-31 Review Article

DOI: 10.1039/D3NP00005B

Correction: Hot off the Press

2024-01-03 Correction

DOI: 10.1039/D3NP90056H

Ligand-modified eggshells for rapid naked-eye detection and removal of trace level Ni2+ ions

P. Rosaiah, S. Vadivel, Kalaivani Dayanidhi, Mohammad Rezaul Karim, Ibrahim A. Alnaser, Sambasivam Sangaraju, M. Dhananjaya, Sang Woo Joo

2024-01-02 Paper

DOI: 10.1039/D3NJ05321K

Eco-efficient pickering foams: leveraging sugarcane waste-derived cellulose nanofibres

Nasim Amiralian, Sandya S. A. Athukoralalage

2023-11-03 Paper

DOI: 10.1039/D3TA04917E

You might also like

Compound Q&A

How should waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3) be handled?

Waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3...

898825-89-3N-Methoxy-N-methyl-1...
Compound Q&A

How should N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine (CAS: 1318338-47-4) be stored?

N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine should be stored in a tightly sealed c...

1318338-47-4N-(4-Biphenylyl)dibe...
Compound Q&A

What is the market or research trend for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1)?

The market for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1) is...

1713-07-13-Acetamido-5-amino-...
Compound Q&A

How should Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) be stored?

Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) ...

61820-03-9Benzyl 2-O-acetyl-3,...
Compound Q&A

What regulatory guidelines apply to 2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3)?

2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3) is regulated under the Glob...

438050-52-32-Ethylpiperazine di...
Compound Q&A

What regulatory guidelines apply to 1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 119462-56-5)?

1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 11946...

119462-56-51,1'-[1,3-Phenyleneb...
Compound Q&A

Are there alternatives to 5-Fluoro-2-(1-pyrrolidinyl)pyridine (CAS: 1287217-79-1) in synthesis?

Several alternatives can be used in the synthesis of 5-Fluoro-2-(1-pyrrolidinyl)...

1287217-79-15-Fluoro-2-(1-pyrrol...
Compound Q&A

What precautions should be taken when handling 6-Bromoimidazo[1,2-a]pyridin-8-amine (CAS: 676371-00-9)?

When handling 6-Bromoimidazo[1,2-a]pyridin-8-amine, it is important to wear appr...

676371-00-96-Bromoimidazo[1,2-a...
Compound Q&A

Are there alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochloride (CAS: 1049740-22-8) in synthesis?

Alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochlo...

1049740-22-8(2S,4R)-4-(4-Nitrobe...

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.