Design and application of an open tubular capillary reactor for solid-phase permethylation of glycans in glycoprotein
Literature Information
Publication Date
2014-12-24
DOI
10.1039/C4AN01045K
Impact Factor
4.616
Authors
Xiaodi Gao, Lingyi Zhang, Weibing Zhang, Liang Zhao
View Original
Abstract
The permethylation derivatization method for structural analysis of glycans is important for characterizing glycoproteins in the study of glycomics. An open tubular capillary reactor coated with NaOH was designed and constructed for solid-phase permethylation of glycans in glycoproteins. The flow rate, capillary length, and inner diameter of the reactor were optimized. The permethylation rate of the model sample β-cyclodextrin reached 81% with a flow rate of 1 μL min−1 in a 32 cm long capillary reactor (i.d. 500 μm). A trace amount of mucin O-glycans was permethylated by the open tubular reactor under low pressure without interference from the freezing of DMSO. Analysis indicates that using this open tubular reactor is a fast, convenient, and efficient method for the permethylation of protein O-glycan.
Related Literature
Palladium(0) alkyne complexes as active species: a DFT investigation
Mårten Ahlquist, Giancarlo Fabrizi, Sandro Cacchi, Per-Ola Norrby
2005-07-20
Communication
DOI: 10.1039/B507784B
A new simple synthesis of poly(thiophene-methine)s
Md. Badruz Zaman, Dmitrii F. Perepichka
2005-07-20
Communication
DOI: 10.1039/B506138E
Directional control of π-stacked building blocks for crystal engineering: the 1,8-naphthalimide synthon
Daniel L. Reger, J. Derek Elgin, Radu F. Semeniuc, Perry J. Pellechia, Mark D. Smith
2005-07-14
Communication
DOI: 10.1039/B504998A
A facile route to fabrication of inorganic–small organic molecule cable-like nanocomposite arrays
Weigang Ju, Mingming Gu, Xiangmin Meng, Wensheng Shi, Xiaohong Zhang, Shuittong Lee
2005-07-21
Communication
DOI: 10.1039/B506459G
PNA C–C+i-motif: superior stability of PNA TC8 tetraplexes compared to DNA TC8 tetraplexes at low pH
Nagendra K. Sharma, Krishna N. Ganesh
2005-07-13
Communication
DOI: 10.1039/B506870C
Evidence of carbon–carbon bond formation on GaAs(100) via Fischer–Tropsch methyleneinsertion reaction mechanism
Neil T. Kemp, Nagindar K. Singh
2005-08-02
Communication
DOI: 10.1039/B506195D
Shape-dependent magnetic properties of low-dimensional nanoscale Prussian blue (PB) analogue SmFe(CN)6·4H2O
Hao-Ling Sun, Hongtao Shi, Fei Zhao, Limin Qi, Song Gao
2005-07-28
Communication
DOI: 10.1039/B507240A
Photosensitizing properties of 2,4-dichlorobenzoic acid and chlorinated biphenyl carboxylic acids, potentially key components of chromophoric dissolved organic matter
Anne L. Boreen, Kristopher McNeill
2005-07-14
Communication
DOI: 10.1039/B506971H
(Z)-Selective cross-dimerization of arylacetylenes with silylacetylenes catalyzed by vinylideneruthenium complexes
Hiroyuki Katayama, Hiroshi Yari, Masaki Tanaka, Fumiyuki Ozawa
2005-07-28
Communication
DOI: 10.1039/B504436G
Lower rim mono-functionalization of resorcinarenes
Frank Hauke, Andrew J. Myles, Julius Rebek Jr.
2005-07-25
Communication
DOI: 10.1039/B506048F
You might also like
Compound Q&A
How should waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3) be handled?
Waste containing N-Methoxy-N-methyl-1,3-thiazole-5-carboxamide (CAS: 898825-89-3...
898825-89-3N-Methoxy-N-methyl-1...
Compound Q&A
How should N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine (CAS: 1318338-47-4) be stored?
N-(4-Biphenylyl)dibenzo[b,d]furan-4-amine should be stored in a tightly sealed c...
1318338-47-4N-(4-Biphenylyl)dibe...
Compound Q&A
What is the market or research trend for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1)?
The market for 3-Acetamido-5-amino-2,4,6-triiodobenzoic acid (CAS: 1713-07-1) is...
1713-07-13-Acetamido-5-amino-...
Compound Q&A
How should Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) be stored?
Benzyl 2-O-acetyl-3,4,6-tri-O-benzyl-beta-D-galactopyranoside (CAS: 61820-03-9) ...
61820-03-9Benzyl 2-O-acetyl-3,...
Compound Q&A
What regulatory guidelines apply to 2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3)?
2-Ethylpiperazine dihydrochloride (CAS: 438050-52-3) is regulated under the Glob...
438050-52-32-Ethylpiperazine di...
Compound Q&A
What regulatory guidelines apply to 1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 119462-56-5)?
1,1'-[1,3-Phenylenebis(methylene)]bis(3-methyl-1H-pyrrole-2,5-dione) (CAS: 11946...
119462-56-51,1'-[1,3-Phenyleneb...
Compound Q&A
Are there alternatives to 5-Fluoro-2-(1-pyrrolidinyl)pyridine (CAS: 1287217-79-1) in synthesis?
Several alternatives can be used in the synthesis of 5-Fluoro-2-(1-pyrrolidinyl)...
1287217-79-15-Fluoro-2-(1-pyrrol...
Compound Q&A
What precautions should be taken when handling 1-((2R,3R,4R,5R)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-hydroxy-3-methoxytetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (CAS: 153631-19-7)?
Proper personal protective equipment (PPE) must be worn when handling this compo...
153631-19-71-((2R,3R,4R,5R)-5-(...
Compound Q&A
What precautions should be taken when handling 6-Bromoimidazo[1,2-a]pyridin-8-amine (CAS: 676371-00-9)?
When handling 6-Bromoimidazo[1,2-a]pyridin-8-amine, it is important to wear appr...
676371-00-96-Bromoimidazo[1,2-a...
Compound Q&A
Are there alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochloride (CAS: 1049740-22-8) in synthesis?
Alternatives to (2S,4R)-4-(4-Nitrobenzyl)pyrrolidine-2-carboxylic acid hydrochlo...
1049740-22-8(2S,4R)-4-(4-Nitrobe...
Source Journal
Analyst
CiteScore:
7.8
Self-citation Rate:
5.6%
Articles per Year:
653
Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.
Recommended Compounds
N1,N1-Bis(4-aminophenyl)benzene-1,4-diamine trihydrochloride
CAS Number:
114254-48-7
Molecular Formula:
C18H21Cl3N4
1-(3-Fluorophenyl)-1H-1,2,4-triazole-3-carboxylic acid
CAS Number:
1292369-51-7
Molecular Formula:
C9H6FN3O2
Recommended Suppliers
Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.