Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays

Literature Information

Publication Date 2007-08-22
DOI 10.1039/B709817K
Impact Factor 6.222
Authors

Shunxing Su, Carlos D. M. Filipe, Robert Pelton


View Original

Abstract

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA–microgel conjugates can be explored for the development of DNA based bioassays and biosensors.

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Contents

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DOI: 10.1039/C0CP90158J

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Chemical Communications

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