Squalene/polyethylenimine based non-viral vectors: synthesis and use in systems for sustained gene release

Literature Information

Publication Date 2018-01-25
DOI 10.1039/C7PY01720K
Impact Factor 5.582
Authors

Geta David, Lilia Clima, Manuela Calin, Cristina Ana Constantinescu, Mihaela Balan-Porcarasu


View Original

Abstract

The design of new non-viral gene vectors and their integration into a biomaterial scaffold are intensively explored nowadays, aimed at developing more efficient gene delivery systems capable of clinical translation. Herein, a series of squalene/branched polyethylenimine (Sq/BPEI) based conjugates were synthesized and their potential in gene delivery, as such or included in a three-dimensional hybrid scaffold (atelocollagen-glycosaminoglycan-polycaprolactone/nano-hydroxyapatite), was investigated. The developed materials were characterized through spectral (1H-NMR, 13C-NMR, FT-IR) and optical (STEM) techniques. The extent and duration of gene expression for the developed systems were monitored using luciferase assays and fluorescence microscopy. All the investigated systems exhibited a high transfection efficiency without or with moderate cytotoxic side effects (MTS assay data). The best results were obtained with the guanidinylated compound Sq-BPEI-G for both simple and combined polyplex/matrix systems. The polyplex delivery from the hybrid matrix was assessed through kinetic studies using a labeled Sq/BPEI conjugate. For the polyplex (Sq/BPEI – DNA plasmid)/3D matrix system, the expression of the delivered plasmids was observed over a 26 day- period. The reported preliminary data recommend the studied biomaterials as possible candidates for the development of a new gene-activated matrix (GAM) platform.

Related Literature

Development of polycarbonate-containing block copolymers for thin film self-assembly applications

Ankit Vora, Rudy J. Wojtecki, Kristin Schmidt, Anindarupa Chunder, Joy Y. Cheng, Alshakim Nelson, Daniel P. Sanders

2015-12-10 Paper

DOI: 10.1039/C5PY01846C

Micelles from self-assembled double-hydrophilic PHEMA-glycopolymer-diblock copolymers as multivalent scaffolds for lectin binding

H. Park, S. Walta, R. R. Rosencrantz, A. Körner, C. Schulte, L. Elling, W. Richtering, A. Böker

2015-12-02 Paper

DOI: 10.1039/C5PY00797F

Contents list

Front/Back Matter

DOI: 10.1039/C6PY90005D

Saturated and stabilized white electroluminescence with simultaneous three-color emission from a six-armed star-shaped single-polymer system

Yuan-Da Jiu, Cheng-Fang Liu, Jian-Yun Wang, Yi Jiang, Wei-Dong Xu, Xin-Wen Zhang

2015-09-28 Paper

DOI: 10.1039/C5PY01480H

Synthesis of thermally cleavable multisegmented polystyrene by an atom transfer nitroxide radical polymerization (ATNRP) mechanism

Wenguang Song, Jian Huang, Cheng Hang, Chenyan Liu, Xuepu Wang, Guowei Wang

2015-09-28 Paper

DOI: 10.1039/C5PY01493J

Chemical vapour deposition of soluble poly(p-xylylene) copolymers with tuneable properties

Ilka E. Paulus, Andreas Greiner

2015-10-07 Paper

DOI: 10.1039/C5PY01343G

UV-Vis/FT-NIR in situ monitoring of visible-light induced polymerization of PEGDA hydrogels initiated by eosin/triethanolamine/O2

Kaja Kaastrup, Chen Wang, Christopher N. Bowman

2015-11-13 Paper

DOI: 10.1039/C5PY01528F

Cheap and fast: oxalic acid initiated CO2-based polyols synthesized by a novel preactivation approach

Yusheng Qin, Lijun Qiao, Yuyang Miao, Xianhong Wang, Fosong Wang

2015-10-12 Paper

DOI: 10.1039/C5PY01338K

You might also like

Compound Q&A

What are the main uses of 4-Nitrophenyl phosphate disodium salt hexahydrate (CAS: 333338-18-4)?

4-Nitrophenyl phosphate disodium salt hexahydrate is primarily used as a substra...

333338-18-44-Nitrophenyl phosph...
Compound Q&A

What are the main uses of 2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4)?

2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4) is widely ...

1060816-01-42-(Trifluoromethyl)-...
Compound Q&A

How should 2-Fluoro-4-biphenylcarboxylic acid (CAS: 137045-30-8) be stored?

2-Fluoro-4-biphenylcarboxylic acid should be stored in a cool, dry place at room...

137045-30-82-Fluoro-4-biphenylc...
Compound Q&A

What industries use Prednisolone-21-Carboxylic Acid (CAS: 61549-70-0)?

Prednisolone-21-Carboxylic Acid is primarily used in the pharmaceutical industry...

61549-70-0Prednisolone-21-Carb...
Compound Q&A

How should 4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) be stored?

4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) should be stored in a co...

3614-72-04-(Hydrazinomethyl)-...
Compound Q&A

What industries use 4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8)?

4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8) i...

92534-70-84-Amino-1-methyl-1H-...
Compound Q&A

What regulatory guidelines apply to dehydropachymic acid (CAS: 77012-31-8)?

Dehydropachymic acid (CAS: 77012-31-8) is regulated by various agencies. It fall...

77012-31-8Dehydropachymic acid
Compound Q&A

What is the market or research trend for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic acid (CAS: 898561-66-5)?

The market and research trends for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic aci...

898561-66-56-[(2,2-Dimethylprop...
Compound Q&A

How should 1,10-Phenanthroline-2,9-dicarbaldehyde (CAS: 57709-62-3) be stored?

1,10-Phenanthroline-2,9-dicarbaldehyde should be stored in a cool, dry place awa...

57709-62-31,10-Phenanthroline-...
Compound Q&A

How is 5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate (CAS: 113952-21-9) typically synthesized?

5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate can be synt...

113952-21-95-Carbamoyl-11-oxo-1...

Source Journal

Polymer Chemistry

Polymer Chemistry
CiteScore: 8.6
Self-citation Rate: 7.3%
Articles per Year: 457

Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.