Squalene/polyethylenimine based non-viral vectors: synthesis and use in systems for sustained gene release
Literature Information
Geta David, Lilia Clima, Manuela Calin, Cristina Ana Constantinescu, Mihaela Balan-Porcarasu
The design of new non-viral gene vectors and their integration into a biomaterial scaffold are intensively explored nowadays, aimed at developing more efficient gene delivery systems capable of clinical translation. Herein, a series of squalene/branched polyethylenimine (Sq/BPEI) based conjugates were synthesized and their potential in gene delivery, as such or included in a three-dimensional hybrid scaffold (atelocollagen-glycosaminoglycan-polycaprolactone/nano-hydroxyapatite), was investigated. The developed materials were characterized through spectral (1H-NMR, 13C-NMR, FT-IR) and optical (STEM) techniques. The extent and duration of gene expression for the developed systems were monitored using luciferase assays and fluorescence microscopy. All the investigated systems exhibited a high transfection efficiency without or with moderate cytotoxic side effects (MTS assay data). The best results were obtained with the guanidinylated compound Sq-BPEI-G for both simple and combined polyplex/matrix systems. The polyplex delivery from the hybrid matrix was assessed through kinetic studies using a labeled Sq/BPEI conjugate. For the polyplex (Sq/BPEI – DNA plasmid)/3D matrix system, the expression of the delivered plasmids was observed over a 26 day- period. The reported preliminary data recommend the studied biomaterials as possible candidates for the development of a new gene-activated matrix (GAM) platform.
Related Literature
Development and application of a universal extraction-free reagent based on an algal glycolipid
Minli Qiu, Jun Cheng, Huajun Zhou, Feihu Che, Yan Hu, Yinghui He, Yuzhu Dai, Yingjie Zhang
DOI: 10.1039/D3AY01246H
Genetically engineered probiotics for an optical imaging-guided tumor photothermal therapy/immunotherapy
Xue Chen, Puze Li, Shiqiang Xie
DOI: 10.1039/D3BM01227A
PVA-based bulk microneedles capable of high insulin loading and pH-triggered degradation for multi-responsive and sustained hypoglycemic therapy
Yuhong Ma, Wei Wang, Mujiao He, Yunzhu Liu, Caihua Li, Yinan Zhong, Quanmin Bu, Dechun Huang, Hongliang Qian, Wei Chen
DOI: 10.1039/D3BM01760E
Milk exosomes anchored with hydrophilic and zwitterionic motifs enhance mucus permeability for applications in oral gene delivery
Chenzhen Zhang, Hengli Zhang, Héctor A. Millán Cotto, Timothy L. Boyer, Matthew R. Warren, Chia-Ming Wang, Joshua Luchan, Pradeep K. Dhal, Rebecca L. Carrier, Ambika G. Bajpayee
DOI: 10.1039/D3BM01089A
A C4-modified bipyridinium multi-mode stationary phase for reversed phase, hydrophilic interaction and ion exchange chromatography
Yan Li, Zelong Long, Lingling Han, Kaijun Quan, Jia Chen, Xiuhui Liu, Hongdeng Qiu
DOI: 10.1039/D3AY01796F
Platelet membrane-based biochemotactic-targeting nanoplatform combining PDT with EGFR inhibition therapy for the treatment of breast cancer
Guoyun Wan, Xuheng Chen, Ruiling Gou, Chenguang Guan, Jiayu Chen, Qian Wang, Qiqing Zhang, Haijiao Wang
DOI: 10.1039/D3BM01627G
Optimization of heteronuclear ultrafast 2D NMR for the study of complex mixtures hyperpolarized by dynamic nuclear polarization
Clément Praud, Victor Ribay, Arnab Dey, Benoît Charrier, Joris Mandral, Jonathan Farjon, Jean-Nicolas Dumez, Patrick Giraudeau
DOI: 10.1039/D3AY01681A
Determination of polyfluoroalkyl substances in cosmetic products using dispersed liquid–liquid extraction coupled with UHPLC-MS/MS
Wenyao Liang, Zemin Xia, Jianhua Tan
DOI: 10.1039/D3AY01553J
Catechol-tetraethylenepentamine co-deposition modified cellulose filter paper for α-glucosidase immobilization and inhibitor screening from traditional Chinese medicine
Guang-Zhen Wan, Chun-Lin Zhang, Juan Chen
DOI: 10.1039/D3AY01835K
Oxygen and pH responsive theragnostic liposomes for early-stage diagnosis and photothermal therapy of solid tumours
Siyi Li, Qinglin Wang, Yingying Ren, Pengtao Bao, Shanyue Guan, Xiaochen Qiu
DOI: 10.1039/D3BM01514A
You might also like
What are the main uses of 4-Nitrophenyl phosphate disodium salt hexahydrate (CAS: 333338-18-4)?
4-Nitrophenyl phosphate disodium salt hexahydrate is primarily used as a substra...
What are the main uses of 2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4)?
2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4) is widely ...
How should 2-Fluoro-4-biphenylcarboxylic acid (CAS: 137045-30-8) be stored?
2-Fluoro-4-biphenylcarboxylic acid should be stored in a cool, dry place at room...
What industries use Prednisolone-21-Carboxylic Acid (CAS: 61549-70-0)?
Prednisolone-21-Carboxylic Acid is primarily used in the pharmaceutical industry...
How should 4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) be stored?
4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) should be stored in a co...
What industries use 4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8)?
4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8) i...
What regulatory guidelines apply to dehydropachymic acid (CAS: 77012-31-8)?
Dehydropachymic acid (CAS: 77012-31-8) is regulated by various agencies. It fall...
What is the market or research trend for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic acid (CAS: 898561-66-5)?
The market and research trends for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic aci...
How should 1,10-Phenanthroline-2,9-dicarbaldehyde (CAS: 57709-62-3) be stored?
1,10-Phenanthroline-2,9-dicarbaldehyde should be stored in a cool, dry place awa...
How is 5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate (CAS: 113952-21-9) typically synthesized?
5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate can be synt...
Source Journal
Polymer Chemistry

Polymer Chemistry welcomes submissions in all areas of polymer science that have a strong focus on macromolecular chemistry. Manuscripts may cover a broad range of fields, yet no direct application focus is required.














![[(1S,2S,3R,4S,7R,9S,10S,12R,15S)-4,12-Diacetyloxy-15-[(2R,3S)-3-benzamido-3-phenyl-2-(2,2,2-trichloroethoxycarbonyloxy)propanoyl]oxy-1,9-dihydroxy-10,14,17,17-tetramethyl-11-oxo-6-oxatetracyclo[11.3.1.03,10.04,7]heptadec-13-en-2-yl] benzoate structure [(1S,2S,3R,4S,7R,9S,10S,12R,15S)-4,12-Diacetyloxy-15-[(2R,3S)-3-benzamido-3-phenyl-2-(2,2,2-trichloroethoxycarbonyloxy)propanoyl]oxy-1,9-dihydroxy-10,14,17,17-tetramethyl-11-oxo-6-oxatetracyclo[11.3.1.03,10.04,7]heptadec-13-en-2-yl] benzoate structure](https://static.chemtradehub.com/structs/100/100431-55-8-7104.webp)