A lab-on-a-chip for monolith-based preconcentration and electrophoresis separation of phosphopeptides

Literature Information

Publication Date 2017-01-18
DOI 10.1039/C6AN02324J
Impact Factor 4.616
Authors

Monica Araya-Farias, Benjamin Carbonnier, Mohamed Guerrouache, Ichraf Ayed, Nacera Aboud, Myriam Taverna, N. Thuy Tran


View Original

Abstract

A microdevice combining online preconcentration and separation of phosphopeptides was developed in a glass microchip. An ethylene glycol methacrylate phosphate (EGMP), acrylamide (AM) and bisacrylamide (BAA) based monolith was synthesized within microchannels through a photo-driven process. Morphological investigations revealed a homogeneous monolithic structure composed of uniform nodules (∼0.8 μm), with a large pore volume (0.62 cm3 g−1) and sufficiently high specific surface area (34.1 m2 g−1). These features make the monolith particularly interesting for preconcentration purposes. Immobilization of Zr4+ ions on the phosphate groups present at the poly(EGMP-co-AM-co-BAA) monolith surface leads to immobilized metal affinity chromatography support. This monolith-Zr4+ showed a great capacity to capture phosphopeptides. Successful preconcentration and separation of a mixture of ERK2 derived peptides differing only by their phosphorylation degree and sites could be achieved with signal enhancement factors between 340 and 910 after only 7 min of preconcentration. This integrated microdevice represents a novel approach for phosphoproteomic applications.

Related Literature

Van der Waals heterostructure of graphene and germanane: tuning the ohmic contact by electrostatic gating and mechanical strain

A. Bafekry, S. Karbasizadeh, M. Faraji, A. Bagheri Khatibani, I. Abdolhosseini Sarsari, D. Gogova, M. Ghergherehchi

2021-10-06 Correction

DOI: 10.1039/D1CP90197D

The growth of a large GdPO4 crystal guided by theoretical simulation and the study of its phonon properties

Xinghong Gong, Yujin Chen, Jianhua Huang, Yanfu Lin, Zundu Luo, Shuiquan Deng, Yidong Huang

2021-09-16 Paper

DOI: 10.1039/D1CP02977K

Polymer network formation mechanism of multifunctional poly(ethylene glycol)s in ionic liquid electrolyte with a lithium salt

Asumi Ishikawa, Namie Ikeda, Shuichi Maeda, Kenta Fujii

2021-07-27 Paper

DOI: 10.1039/D1CP02710G

Enhanced photocatalytic activity, transport properties and electronic structure of Mn doped GdFeO3 synthesized using the sol–gel process

Ritwik Maity, Alo Dutta, Saswata Halder, Santiranjan Shannigrahi, Kalyan Mandal, T. P. Sinha

2021-07-22 Paper

DOI: 10.1039/D1CP00621E

Core-softened water–alcohol mixtures: the solute-size effects

Murilo S. Marques, Vinicius F. Hernandes, José Rafael Bordin

2021-07-05 Paper

DOI: 10.1039/D1CP00751C

Unravelling the nature of a toluene–fumaronitrile complex

Andrzej J. Kałka, Mateusz Z. Brela, Andrzej M. Turek

2021-07-03 Paper

DOI: 10.1039/D1CP01895G

Bishop's hat silicene: a planar square silicon bilayer decorated with adatoms

Pedro Borlido, Miguel A. L. Marques, Silvana Botti

2021-08-02 Paper

DOI: 10.1039/D1CP01316E

Understanding charge storage in Nb2CTx MXene as an anode material for lithium ion batteries

Tao Hu, Zuohua Wang, Weizhen Wang, Yan Liang, Chao Zhang, Cuiyu Li, Hailong Wang, Hongxia Lu, Zhiqing Yang, Hongwang Zhang, Xiaohui Wang

2021-09-16 Paper

DOI: 10.1039/D1CP03070A

Noncentrosymmetric Weyl phase and topological phase transition in bulk MoTe

Jia-Fang Wu, Sha-Sha Ke, Yong Guo, Huai-Wu Zhang

2021-09-25 Paper

DOI: 10.1039/D1CP02793J

Scouting for strong light–matter coupling signatures in Raman spectra

Lukasz Piatkowski, Frank Wackenhut, Sylwester Gawinkowski, Alfred J. Meixner

2021-07-29 Paper

DOI: 10.1039/D1CP01863A

You might also like

Compound Q&A

What precautions should be taken when handling 4-Methyl-6-(trifluoromethyl)quinoline (CAS: 40716-16-3)?

When handling 4-Methyl-6-(trifluoromethyl)quinoline (CAS: 40716-16-3), safety go...

40716-16-34-Methyl-6-(trifluor...
Compound Q&A

What is 4-(3,5-Difluorophenyl)aniline (CAS: 405058-00-6)?

4-(3,5-Difluorophenyl)aniline is an aromatic organic compound with the CAS numbe...

405058-00-64-(3,5-Difluoropheny...
Compound Q&A

How is 5-{[4-(Trifluoromethyl)phenyl]sulfanyl}-1,2,3-thiadiazole-4-carboxylic acid (CAS: 338982-07-3) typically synthesized?

5-{[4-(Trifluoromethyl)phenyl]sulfanyl}-1,2,3-thiadiazole-4-carboxylic acid can ...

338982-07-35-{[4-(Trifluorometh...
Compound Q&A

What is the market or research trend for 4-Benzylaniline hydrochloride (CAS: 6317-57-3)?

The market for 4-Benzylaniline hydrochloride (CAS: 6317-57-3) is steadily growin...

6317-57-34-Benzylaniline hydr...
Compound Q&A

Is [3-(Diethylsulfamoyl)phenyl]boronic acid (CAS: 871329-58-7) safe?

[3-(Diethylsulfamoyl)phenyl]boronic acid is generally considered safe when handl...

871329-58-7[3-(Diethylsulfamoyl...
Compound Q&A

What are the main uses of 3-Bromo-2,5-dimethoxyaniline (CAS: 115929-62-9)?

3-Bromo-2,5-dimethoxyaniline is mainly used in the pharmaceutical and chemical i...

115929-62-93-Bromo-2,5-dimethox...
Compound Q&A

What regulatory guidelines apply to N-Methyl-1-(5-methyl-1H-indol-3-yl)methanamine (CAS: 915922-67-7)?

N-Methyl-1-(5-methyl-1H-indol-3-yl)methanamine (CAS: 915922-67-7) is subject to ...

915922-67-7N-Methyl-1-(5-methyl...
Compound Q&A

What industries use Carbamic acid, N-[(5S)-5,6-diamino-6-oxohexyl]-, 1,1-dimethylethyl ester (CAS: 24828-96-4)?

This compound is primarily used in the pharmaceutical industry for the synthesis...

24828-96-4Carbamic acid, N-[(5...
Compound Q&A

How should 2-Methyl-2-propanyl [(1S,3R)-3-aminocyclohexyl]carbamate (CAS: 1298101-47-9) be stored?

2-Methyl-2-propanyl [(1S,3R)-3-aminocyclohexyl]carbamate (CAS: 1298101-47-9) sho...

1298101-47-92-Methyl-2-propanyl ...
Compound Q&A

What industries use Ethyl 2-bromo-4,4,4-trifluorobutanoate (CAS: 367-33-9)?

Ethyl 2-bromo-4,4,4-trifluorobutanoate (CAS: 367-33-9) is utilized in the pharma...

367-33-9Ethyl 2-bromo-4,4,4-...

Source Journal

Analyst

Analyst
CiteScore: 7.8
Self-citation Rate: 5.6%
Articles per Year: 653

Analyst publishes analytical and bioanalytical research that reports premier fundamental discoveries and inventions, and the applications of those discoveries, unconfined by traditional discipline barriers.

Recommended Compounds

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.