A new strategy to prepare giant vesicles from surface active ionic liquids (SAILs): a study of protein dynamics in a crowded environment using a fluorescence correlation spectroscopic technique

Literature Information

Publication Date 2016-04-12
DOI 10.1039/C5CP07225E
Impact Factor 3.676
Authors

Chiranjib Banerjee, Arpita Roy, Niloy Kundu, Debasis Banik, Nilmoni Sarkar


View Original

Abstract

A simple procedure for the preparation of giant vesicles using surface active ionic liquids (SAILs) has been provided in this paper. SAILs, used to form vesicles, were synthesized by replacing the cationic part of Aerosol OT (AOT) with cations having alkyl chains of different lengths (ammonium and imidazolium cations). The number of carbons in the alkyl chains of the cations was varied from eight to sixteen. From the observed results, the formation of giant vesicles is found to be dependent on the alkyl chain length as well as the organic moieties of the respective cations. These giant vesicles were characterized using fluorescence lifetime imaging microscopy (FLIM). The conformational dynamics of bovine serum albumin (BSA) inside these giant vesicles was determined using fluorescence correlation spectroscopy (FCS) to get an idea about the protein dynamics in a constrained environment. The interaction of the giant vesicles with the protein was confirmed by the change in the diffusion coefficient and the conformational fluctuation time.

Related Literature

Contents list

Front/Back Matter

DOI: 10.1039/C9CP90090J

Tuning of the gold work function by carborane films studied using density functional theory

Martin Hladík, Aliaksei Vetushka, Antonín Fejfar, Héctor Vázquez

2019-02-22 Paper

DOI: 10.1039/C9CP00346K

Comparison of hydrogen vacancies in KDP and ADP crystals: a combination of density functional theory calculations and experiment

Tingting Sui, Yafei Lian, Mingxia Xu, Lisong Zhang, Yanlu Li, Xian Zhao, Xinguang Xu, Xun Sun

2019-02-22 Paper

DOI: 10.1039/C8CP07685E

Kinetics of the reactions of NO3 radical with alkanes

Li Zhou, Mahmoud Idir, Véronique Daële, Abdelwahid Mellouki

2019-02-05 Paper

DOI: 10.1039/C8CP07675H

Tritium trapping and migration mechanisms in Li2O: a first-principles study

Xin Xiang, Guikai Zhang, Feilong Yang, Tao Tang

2019-02-13 Paper

DOI: 10.1039/C8CP07187J

How intermolecular interactions influence electronic absorption spectra: insights from the molecular packing of uracil in condensed phases

Fangjia Fu, Kang Liao, Jing Ma, Zheng Cheng, Dong Zheng, Liuzhou Gao, Chungen Liu, Shuhua Li, Wei Li

2019-01-16 Paper

DOI: 10.1039/C8CP06152A

Structural studies of spray pyrolysis synthesized oxygen deficient anatase TiO2 thin films by using X-ray absorption spectroscopy

Madhusmita Sahoo, A. K. Yadav, Subrata Ghosh, S. N. Jha, D. Bhattacharyya, Tom Mathews

2019-02-18 Paper

DOI: 10.1039/C8CP06811A

The study of rhenium pentacarbonyl complexes using single-atom chemistry in the gas phase

Yang Wang, Shiwei Cao, Fangli Fan, Jie Yang, Hiromitsu Haba, Yukiko Komori, Takuya Yokokita, Kouji Morimoto, Daiya Kaji, Andreas Türler

2019-03-07 Paper

DOI: 10.1039/C8CP07844K

You might also like

Compound Q&A

What are the main uses of 4-Nitrophenyl phosphate disodium salt hexahydrate (CAS: 333338-18-4)?

4-Nitrophenyl phosphate disodium salt hexahydrate is primarily used as a substra...

333338-18-44-Nitrophenyl phosph...
Compound Q&A

What are the main uses of 2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4)?

2-(Trifluoromethyl)-1,3-oxazole-4-carboxylic Acid (CAS: 1060816-01-4) is widely ...

1060816-01-42-(Trifluoromethyl)-...
Compound Q&A

How should 2-Fluoro-4-biphenylcarboxylic acid (CAS: 137045-30-8) be stored?

2-Fluoro-4-biphenylcarboxylic acid should be stored in a cool, dry place at room...

137045-30-82-Fluoro-4-biphenylc...
Compound Q&A

What industries use Prednisolone-21-Carboxylic Acid (CAS: 61549-70-0)?

Prednisolone-21-Carboxylic Acid is primarily used in the pharmaceutical industry...

61549-70-0Prednisolone-21-Carb...
Compound Q&A

How should 4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) be stored?

4-(Hydrazinomethyl)-1,2,3-benzenetriol (CAS: 3614-72-0) should be stored in a co...

3614-72-04-(Hydrazinomethyl)-...
Compound Q&A

What industries use 4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8)?

4-Amino-1-methyl-1H-pyrazole-5-carboxylic acid hydrochloride (CAS: 92534-70-8) i...

92534-70-84-Amino-1-methyl-1H-...
Compound Q&A

What regulatory guidelines apply to dehydropachymic acid (CAS: 77012-31-8)?

Dehydropachymic acid (CAS: 77012-31-8) is regulated by various agencies. It fall...

77012-31-8Dehydropachymic acid
Compound Q&A

What is the market or research trend for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic acid (CAS: 898561-66-5)?

The market and research trends for 6-[(2,2-Dimethylpropanoyl)amino]nicotinic aci...

898561-66-56-[(2,2-Dimethylprop...
Compound Q&A

How should 1,10-Phenanthroline-2,9-dicarbaldehyde (CAS: 57709-62-3) be stored?

1,10-Phenanthroline-2,9-dicarbaldehyde should be stored in a cool, dry place awa...

57709-62-31,10-Phenanthroline-...
Compound Q&A

How is 5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate (CAS: 113952-21-9) typically synthesized?

5-Carbamoyl-11-oxo-10,11-dihydro-5H-dibenzo[b,f]azepin-10-yl acetate can be synt...

113952-21-95-Carbamoyl-11-oxo-1...

Source Journal

Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
Articles per Year: 3036

Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

Recommended Suppliers

Disclaimer
This page provides academic journal information for reference and research purposes only. We are not affiliated with any journal publishers and do not handle publication submissions. For publication-related inquiries, please contact the respective journal publishers directly.
If you notice any inaccuracies in the information displayed, please contact us at support@chemtradehub.com. We will promptly review and address your concerns.