A microfabrication-free nanoliter droplet array for nucleic acid detection combined with isothermal amplification

Literature Information

Publication Date 2015-05-05
DOI 10.1039/C5AN00573F
Impact Factor 4.616
Authors

Weiwei Xu, Chao Chen, Zuhong Lu, Jiong Li


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Abstract

A nanoliter droplet array based on a hydrophilic–hydrophobic patterned chip is developed without using microfabrication technology. Combined with the isothermal amplification technology, it has been applied to perform nucleic acid detection, showing excellent specificity and sensitivity. As a versatile platform, it is used to detect three gene targets successfully.

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