A simple assay for glutathione in whole blood

Literature Information

Publication Date 2015-04-17
DOI 10.1039/C5AN00345H
Impact Factor 4.616
Authors

Lovemore Hakuna, Brandon Doughan, Jorge O. Escobedo, Robert M. Strongin


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Abstract

A method for detecting glutathione selectively in whole blood deposited on filter paper is described. GSH is fractionated from proteins, hemoglobin and other potentially interfering components and determined using a resorufin-acrylate fluorescent probe. The relative standard deviation is lower than 5% (n = 5). Recoveries of GSH from whole blood are between 94% and 108.6%.

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