On-line visualization of multicolor chemical images with stimulated Raman scattering spectral microscopy

Literature Information

Publication Date 2015-03-30
DOI 10.1039/C5AN00335K
Impact Factor 4.616
Authors

Yoichi Otsuka, Koji Makara, Shuya Satoh, Hiroyuki Hashimoto, Yasuyuki Ozeki


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Abstract

We demonstrate multicolor, on-line visualization in label-free biomedical microscopy based on stimulated Raman scattering (SRS). Fast data acquisition of SRS spectral images and subsequent image generation are achieved. The loading vectors for the blind separation of chemical components are predetermined by multivariate analysis at a certain field of view (FOV) and are applied to execute on-line visualization of chemical images at other FOVs. We also show that the response time can be shortened by reducing the number of spectral data points. This on-line visualization system is expected to increase the usability of the Raman imaging system and the analytical throughput for screening.

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