Direct electrochemical characterization of the interaction between haemoglobin and nitric oxide

Literature Information

Publication Date 2000-09-07
DOI 10.1039/B005527L
Impact Factor 3.676
Authors

Chunhai Fan, Xiaocheng Chen, Genxi Li, Jianqin Zhu, Dexu Zhu, Hugo Scheer


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Abstract

Direct electrochemistry was carried out to investigate the interaction between haemoglobin (Hb) and nitric oxide (NO), employing a Hb–DNA film modified electrode. DNA was used as an electrochemical “promoter” for the direct electron transfer of Hb at the electrode surface. Aside from a pair of peaks corresponding to the redox reaction of Hb, two additional peaks were observed in a solution containing NO. The pre-peak, located at −0.070 V s. SCE, was proposed to come from the chemical redox reaction, i.e., NO-induced oxidation of Hb in the film, coupled electron transfer process of Hb. The other peak, at −0.684 V s. SCE, was assigned to the reduction of heme-ligated NO. These phenomena were proposed to be related to the electron transfer and binding process between Hb and NO.

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Physical Chemistry Chemical Physics

Physical Chemistry Chemical Physics
CiteScore: 5.5
Self-citation Rate: 10.3%
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Physical Chemistry Chemical Physics (PCCP) is an international journal co-owned by 19 physical chemistry and physics societies from around the world. This journal publishes original, cutting-edge research in physical chemistry, chemical physics and biophysical chemistry. To be suitable for publication in PCCP, articles must include significant innovation and/or insight into physical chemistry; this is the most important criterion that reviewers and Editors will judge against when evaluating submissions. The journal has a broad scope and welcomes contributions spanning experiment, theory, computation and data science. Topical coverage includes spectroscopy, dynamics, kinetics, statistical mechanics, thermodynamics, electrochemistry, catalysis, surface science, quantum mechanics, quantum computing and machine learning. Interdisciplinary research areas such as polymers and soft matter, materials, nanoscience, energy, surfaces/interfaces, and biophysical chemistry are welcomed if they demonstrate significant innovation and/or insight into physical chemistry. Joined experimental/theoretical studies are particularly appreciated when complementary and based on up-to-date approaches.

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